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PMID: 6324088 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence of the genes for tRNACys and tRNAAsp from spinach chloroplasts.

Nucleic acids research ·Vol. 11 ·No. 24 ·1983-12-20 ·Pages 8547-54

Holschuh K, Bottomley W, Whitfeld PR

Abstract

We have determined the map location and primary structure of two fragments of spinach chloroplast DNA which encompass the genes for tRNACysGCA and tRNAAspGUC. Identification of the genes for these two RNA species is based on the sequence of their anticodon triplets and on a comparison of the sequences with those of the equivalent tRNAs from Escherichia coli. Each gene occurs only once on the spinach chloroplast genome and neither contains an intervening sequence. Hybridization of the restriction fragments carrying these genes to chloroplast tRNA showed that both genes are transcribed in vivo.

MeSH Terms
Anticodon Base Sequence Chloroplasts/metabolism DNA Restriction Enzymes Genes Nucleic Acid Hybridization Plants/genetics RNA, Transfer, Amino Acyl/genetics Transcription, Genetic
Chemicals
Anticodon RNA, Transfer, Amino Acyl DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Holschuh K
Bottomley W
Whitfeld P R
References (23)
23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.24.8547
Published
1983-12-20
Pages
8547-54
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326606
Subset
IM
Databases
GENBANK
X00216, X00217
External Links
PubMed source DOI: 10.1093/nar/11.24.8547 PMC Full Text View journal details
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