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PMID: 6324093 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcription of a cloned Xenopus laevis H4 histone gene in the homologous frog oocyte system depends on an evolutionary conserved sequence motif in the -50 region.

Nucleic acids research ·Vol. 11 ·No. 24 ·1983-12-20 ·Pages 8641-57

Clerc RG, Bucher P, Strub K, Birnstiel ML

Abstract

A cloned Xenopus laevis H4 histone gene has been expressed in the X.laevis oocyte nucleus. The homologous histone H4 gene can be correctly and efficiently expressed in the frog oocyte even in presence of bacterial vector DNA. As revealed by both analytical gel electrophoresis and S1 mapping, two H4 mRNAs are specified with different transcriptional efficiencies from the tandemly repeated promoter. Results from deletion mapping of the sequences essential for promoting H4 transcription show that drastic reduction of transcription is obtained when the sequences lying between -64 and -35 bp from the mRNA cap site are removed. We demonstrate by DNA sequence comparison using a novel computer program that this important area of the H4 promoter contains two highly conserved DNA motifs near positions -51 to -46 upstream from the cap site in all H4 gene promoters analysed.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Cloning, Molecular DNA Restriction Enzymes Female Genetic Vectors Histones/genetics Mutation Oocytes/metabolism Operon RNA, Messenger/genetics Transcription, Genetic
Chemicals
Histones RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Clerc R G
Bucher P
Strub K
Birnstiel M L
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.24.8641
Published
1983-12-20
Pages
8641-57
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326613
Subset
IM
Databases
GENBANK
X00224
External Links
PubMed source DOI: 10.1093/nar/11.24.8641 PMC Full Text View journal details
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