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PMID: 6324137 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence organization within and flanking clusters of 5S ribosomal RNA genes in Tetrahymena.

Nucleic acids research ·Vol. 12 ·No. 6 ·1984-03-26 ·Pages 3003-21

Pederson DS, Yao MC, Kimmel AR, Gorovsky MA

Abstract

Macro- and micronuclei of Tetrahymena thermophila each contain approximately 30 clusters of 5S genes per haploid genome. Structural changes in DNA sequences associated with some of these clusters occur during the development of the transcriptionally active macronucleus from the transcriptionally inert micronucleus. Exonuclease digestion indicates that DNA fragmentation is not responsible for these changes, making it likely that sequence rearrangements occur near some 5S genes during macronuclear development. These rearrangements appear to be random in location with respect to the 5S genes and do not alter either the tandem repeat organization of the genes, the average number (five) or the range in number (one to about 16) of genes per cluster. The 5S gene clusters are not closely linked and are not flanked by common repeating elements large enough to cross-hybridize. Sequence analysis of one tandem repeat suggests that Tetrahymena 5S genes have intragenic promoters. These observations indicate that features other than DNA rearrangements or DNA sequence per se are responsible for the transcriptional activation of 5S genes during macronuclear development.

MeSH Terms
Animals Base Sequence Cell Nucleus/metabolism Cloning, Molecular DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Genes Haploidy Nucleic Acid Hybridization RNA, Ribosomal/genetics Tetrahymena/genetics
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pederson D S
Yao M C
Kimmel A R
Gorovsky M A
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43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.6.3003
Published
1984-03-26
Pages
3003-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318722
Subset
IM
Databases
GENBANK
X00475
External Links
PubMed source DOI: 10.1093/nar/12.6.3003 PMC Full Text View journal details
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