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PMID: 6326058 Published · ppublish English Journal Article

The nucleotide sequence of the cloned nusA gene and its flanking region of Escherichia coli.

Nucleic acids research ·Vol. 12 ·No. 7 ·1984-04-11 ·Pages 3333-42

Ishii S, Ihara M, Maekawa T, Nakamura Y, Uchida H, Imamoto F

Abstract

The nucleotide sequence of the promoter-proximal portion of the nusA operon including the genes for tRNAMetf2, a 15 kilodalton protein and the initial portion of the nusA gene has been determined previously (1). Here, we report the sequence for the entire nusA gene and its flanking region. The open reading frame, consisting of 1,482 nucleotides, was identified as that of the nusA protein on the basis of agreement of the amino acid sequence deduced from the DNA sequence with the N-terminal sequence of the purified nusA protein. The molecular weight of 54,417 daltons calculated for the 494 amino acid polypeptide is significantly lower than that determined previously by SDS polyacrylamide gel analysis. The nusA gene is immediately followed by another open reading frame encoding a polypeptide of at least 22 amino acids, which was identified as the initial portion of the infB structural gene. In the spacer region of 24 base pairs between the nusA and infB structural genes there is no significant DNA sequence that fits the canonical transcriptional termination signal or promoter sequence. We suggest, therefore, that the genes for tRNAMetf2, a 15 kilodalton protein, the nusA protein and IF2 alpha, aligned in this order, are co-transcribed.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Molecular Weight Operon
Chemicals
Bacterial Proteins DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ishii S
Ihara M
Maekawa T
Nakamura Y
Uchida H
Imamoto F
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.7.3333
Published
1984-04-11
Pages
3333-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318749
Subset
IM
Databases
GENBANK
K01175, X00513
External Links
PubMed source DOI: 10.1093/nar/12.7.3333 PMC Full Text View journal details
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