Home LiteratureArticle Details
PMID: 6294462 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of a recombinant lambda phage carrying nusA and surrounding region of the Escherichia coli K-12 chromosome.

Molecular & general genetics : MGG ·Vol. 187 ·No. 2 ·1982-00-00 ·Pages 248-53

Holowachuk EW, Friesen JD

Abstract

A recombinant bacteriophage lambda, lambda argG-6, has been isolated which carries the argG gene and neighbouring loci on an EcoRI-generated 15.5 Kb DNA fragment from the Escherichia coli chromosome. The locations of the argG, nusA and pnp genes on the 15.5 Kb DNA fragment have been determined. In the case of nusA, a Tn5 insertion and sub-cloning of restriction fragments were used to locate the gene. The gene products of nusA and pnp have been identified on one- and two-dimensional polyacrylamide gels. The clockwise gene order was found to be argG-nusA-pnp.

MeSH Terms
Bacterial Proteins/biosynthesis Bacteriophage lambda/genetics,metabolism Base Sequence Chromosomes, Bacterial DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial/genetics DNA, Recombinant Escherichia coli/genetics Genes, Bacterial Plasmids Polyribonucleotide Nucleotidyltransferase/metabolism
Chemicals
Bacterial Proteins DNA Transposable Elements DNA, Bacterial DNA, Recombinant Polyribonucleotide Nucleotidyltransferase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Holowachuk E W
Friesen J D
References (38)
38 references, click to expand
  1. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. L factor that is required for beta-galactosidase synthesis is the nusA gene product involved in transcription termination.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1991-4 PMID: 6154941
  4. The nus mutations affect transcription termination in Escherichia coli.
    Nature. 1981 Jul 16;292(5820):212-5 PMID: 6265784
  5. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  6. Cloning of E. coli pnp gene from an episome.
    Mol Gen Genet. 1981;183(2):298-305 PMID: 6276682
  7. Autogenous and post-transcriptional regulation of RNA polymerase synthesis.
    Mol Cell Biochem. 1980 Aug 16;31(3):177-96 PMID: 7003354
  8. Organization of genes for transcription and translation in the rif region of the Escherichia coli chromosome.
    J Bacteriol. 1979 Jan;137(1):584-94 PMID: 368032
  9. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  10. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.
    Science. 1977 Apr 8;196(4286):175-7 PMID: 322278
  11. Mapping of ribosomal protein genes by in vitro protein synthesis using DNA fragments of lambdafus3 transducing phage DNA as templates.
    J Biol Chem. 1977 Oct 25;252(20):7365-83 PMID: 332694
  12. Feedback regulation of ribosomal protein synthesis in E. coli: localization of the mRNA target sites for repressor action of ribosomal protein L1.
    Cell. 1981 Apr;24(1):243-9 PMID: 7016337
  13. Genetic characterization of a bacterial locus involved in the activity of the N function of phage lambda.
    Virology. 1974 Mar;58(1):141-8 PMID: 4595374
  14. Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli.
    J Bacteriol. 1979 Jun;138(3):832-8 PMID: 378941
  15. Regulation of ribonucleic acid polymerase synthesis during restriction of an Escherichia coli mutant temperature sensitive for transcription factor sigma.
    J Bacteriol. 1980 Jun;142(3):1049-54 PMID: 6991491
  16. Identification of ribosomal protein S7 as a repressor of translation within the str operon of E. coli.
    Cell. 1981 May;24(2):413-9 PMID: 7016341
  17. Synthesis of ribonucleic acid and protein in plasmid-containing minicells of Escherichia coli K-12.
    J Bacteriol. 1971 Jul;107(1):21-33 PMID: 4935321
  18. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  19. Cloning the spoT gene of Escherichia coli: identification of the spoT gene product.
    J Bacteriol. 1979 Mar;137(3):1100-10 PMID: 374338
  20. The nusA gene protein of Escherichia coli. Its identification and a demonstration that it interacts with the gene N transcription anti-termination protein of bacteriophage lambda.
    J Mol Biol. 1981 Mar 25;147(1):11-23 PMID: 6455533
  21. Characterization of polynucleotide phosphorylase mutants of Escherichia coli.
    J Bacteriol. 1969 Mar;97(3):1437-43 PMID: 4887520
  22. Transcriptional and post-transcriptional control of RNA polymerase and ribosomal protein genes cloned on composite ColE1 plasmids in the bacterium Escherichia coli.
    J Biol Chem. 1979 Aug 25;254(16):7540-7 PMID: 381287
  23. Cooperative effects of bacterial mutations affecting lambda N gene expression. I. Isolation and characterization of a nusB mutant.
    Virology. 1976 Aug;73(1):119-27 PMID: 785802
  24. Transduction of linked genetic characters of the host by bacteriophage P1.
    Virology. 1955 Jul;1(2):190-206 PMID: 13267987
  25. Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids.
    Mol Gen Genet. 1979 May 23;173(1):39-50 PMID: 381841
  26. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  27. Interaction of the sigma factor and the nusA gene protein of E. coli with RNA polymerase in the initiation-termination cycle of transcription.
    Cell. 1981 May;24(2):421-8 PMID: 6263495
  28. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  29. Regulation of RNA polymerase synthesis. Conditional lethal amber mutations in the beta subunit gene.
    J Biol Chem. 1980 Apr 25;255(8):3536-41 PMID: 6988428
  30. Contranscription of genes for RNA polymerase subunits beta and beta' with genes for ribosomal proteins in Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3891-5 PMID: 358203
  31. Termination of transcription by nusA gene protein of Escherichia coli.
    Nature. 1981 Jul 16;292(5820):215-20 PMID: 6265785
  32. Bacteriophage lambda vehicle for the direct cloning of Escherichia coli promoter DNA sequences: feedback regulation of the rplJL-rpoBC operon.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):2124-8 PMID: 6445564
  33. Isolation of the bacteriophage lambda A-gene protein.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):581-5 PMID: 1054839
  34. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  35. Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli.
    J Bacteriol. 1969 Mar;97(3):1431-6 PMID: 4887519
  36. Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B".
    Proc Natl Acad Sci U S A. 1946 May;32(5):120-8 PMID: 16588724
  37. Identification of a single promoter in E. coli for rplJ, rplL and rpoBC.
    Nature. 1978 Nov 2;276(5683):33-7 PMID: 368640
  38. Genetic studies of the ribosomal proteins in Escherichia coli. XI. Mapping of the genes for L21, L27, S15 and S21 by using hybrid bacteria and over-production of these proteins in the merodiploid strains.
    Mol Gen Genet. 1978 Apr 6;160(2):151-5 PMID: 349351
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331126
Published
1982-00-00
Pages
248-53
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331126
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2