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PMID: 6326093 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA binding activity of polyoma virus large tumor antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 7 ·1984-04-00 ·Pages 1941-5

Dilworth SM, Cowie A, Kamen RI, Griffin BE

Abstract

Polyoma virus large tumor antigen from productively infected mouse cells has been purified to greater than 50% homogeneity by a simple immunoaffinity procedure using monoclonal antibodies. A radioimmunoreaction was devised for assaying purity. The purified large tumor antigen retained its antigenicity and its ability to bind DNA specifically. The regions on the polyoma virus genome recognized by the protein were characterized. Three binding regions were localized within the portion of the genome between the viral origin of DNA replication and the protein coding sequence, overlapping the early promoter and the sites of initiation of mRNAs that specify the viral tumor antigens. The binding regions each contain direct repeats of the pentanucleotide sequence G-R-G-G-C.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Base Sequence Cells, Cultured Cloning, Molecular DNA Replication DNA, Viral/genetics Deoxyribonuclease I Embryo, Mammalian Endodeoxyribonucleases Genes Genes, Viral Mice Molecular Weight Polyomavirus/genetics Protein Binding Protein Kinases/metabolism Viral Proteins/isolation & purification,metabolism
Chemicals
Antigens, Polyomavirus Transforming DNA, Viral Viral Proteins Protein Kinases Endodeoxyribonucleases Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dilworth S M
Cowie A
Kamen R I
Griffin B E
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.7.1941
Published
1984-04-00
Pages
1941-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345412
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.7.1941 PMC Full Text View journal details
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