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PMID: 6326109 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression and rearrangement of homologous immunoglobulin VH genes in two mouse strains.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 7 ·1984-04-00 ·Pages 2167-71

Near RI, Juszczak EC, Huang SY, Sicari SA, Margolies MN, Gefter ML

Abstract

A family of murine anti-p-azophenylarsonate (Ars) antibodies share a variable (V) region serologically defined marker, the 36-60 idiotype (Id36-60). Most mouse strains possess five genes highly homologous to the gene encoding the heavy (H) chain V region of antibodies bearing Id36-60 (VH36-60); however, only one of these genes is ever utilized by hybridomas whose antibodies bind Ars and bear Id36-60. The relevant VH genes were cloned from A/J and BALB/c mouse DNA libraries. Their DNA sequences were found to differ at only two positions. Southern blot analysis, protein sequence determination, and nucleic acid sequence determination indicate that the above hybridomas utilize the same joining (JH3), diversity (D), and VH gene segments regardless of BALB/c or A/J strain origin. Despite this virtual identity, BALB/c and A/J mouse strains express quite different serum levels of Id36-60-bearing antibodies when immunized with Ars. The basis of this regulatory process is discussed.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Kidney/immunology Mice Mice, Inbred A Mice, Inbred BALB C Mice, Inbred CBA Nucleic Acid Hybridization Species Specificity
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Variable Region DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Near R I
Juszczak E C
Huang S Y
Sicari S A
Margolies M N
Gefter M L
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.7.2167
Published
1984-04-00
Pages
2167-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345458
Subset
IM
Grants
NIAID NIH HHS · 1 F32 AI06247-01 · United States
NCI NIH HHS · CA-24432 · United States
NHLBI NIH HHS · HL-19259 · United States
Databases
GENBANK
K01569, K01570
External Links
PubMed source DOI: 10.1073/pnas.81.7.2167 PMC Full Text View journal details
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