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PMID: 6308665 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Recombination between antibody heavy chain variable-region genes: evidence for gene conversion.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 16 ·1983-08-00 ·Pages 4997-5001

Krawinkel U, Zoebelein G, Brüggemann M, Radbruch A, Rajewsky K

Abstract

The murine hybridoma line B1-8.delta 1 secretes monoclonal IgD lambda 1 antibodies specific for the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP). The variable (V) region of these antibodies is defined by a characteristic pattern of idiotopes. A spontaneous V-region variant (B1-8.V1) with altered idiotope pattern was selected. The structural variation is confined to the V region of the heavy chain. It was shown previously that the variant V region is encoded by a gene that was generated by a crossover between the rearranged VDJ gene of the wild type (B1-8.delta 1) and a neighboring germ-line VH gene. In the present study the nucleotide sequence of coding and flanking regions of the VH gene expressed in variant B1-8.V1 was determined. Wild-type and variant VH genes differ at 15 positions in a region between leader sequence and codon 66. The sequence of the region carrying the substitutions is identical to the sequence of the corresponding region in a neighboring germ-line VH gene. This implies that the variant VH gene was generated by a mechanism of recombination more complicated than single crossover. Gene conversion as the mechanism of the recombination is discussed.

MeSH Terms
Animals Bacteriophage lambda/genetics Base Sequence Binding Sites, Antibody/genetics Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/analysis Gene Conversion Genes Genotype Hybridomas/immunology Immunoglobulin Heavy Chains/genetics Immunoglobulin Idiotypes/genetics Immunoglobulin Variable Region/genetics Mice Nucleic Acid Hybridization
Chemicals
DNA, Recombinant Immunoglobulin Heavy Chains Immunoglobulin Idiotypes Immunoglobulin Variable Region DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Krawinkel U
Zoebelein G
Brüggemann M
Radbruch A
Rajewsky K
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48 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.16.4997
Published
1983-08-00
Pages
4997-5001
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384175
Subset
IM
Databases
GENBANK
K00684
External Links
PubMed source DOI: 10.1073/pnas.80.16.4997 PMC Full Text View journal details
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