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PMID: 6327291 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Processing of yeast mitochondrial messenger RNAs at a conserved dodecamer sequence.

The EMBO journal ·Vol. 3 ·No. 4 ·1984-04-00 ·Pages 829-34

Osinga KA, De Vries E, Van der Horst G, Tabak HF

Abstract

The yeast mitochondrial genes coding for cytochrome c oxidase subunit I ( COX1 ) and the ATPase subunits 8 and 6 are organized in one transcription unit. Precise mapping of RNA termini with S1 nuclease and primer extension analysis shows that the 3' end of the COX1 mRNA and the 5' end of the ATPase precursor RNA are juxtaposed within a conserved dodecamer sequence (5'- AAUAAUAUUCUU -3'). Sequence comparison reveals that this motif is present downstream of nearly all protein-encoding genes, including extragenic unassigned reading frames ( URFs ) and two URFs located within introns. Also the 3' terminus of an RNA species derived from the URF -containing intron of the large rRNA gene maps within such a dodecamer sequence. It is likely, therefore, that this motif serves as a processing point in the generation of mature mRNA. From a comparison of the various transcription units, we infer that RNAs that originate from an endonucleolytic cleavage at this sequence have stable 3' termini, while further processing of the 5' ends occurs. The efficiency of the initial cleavage varies between the different positions at which the motif is present.

MeSH Terms
Adenosine Triphosphatases/genetics Base Sequence DNA DNA, Fungal/genetics DNA, Mitochondrial/genetics Electron Transport Complex IV/genetics Endonucleases Macromolecular Substances Nucleic Acid Hybridization RNA, Fungal/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
DNA, Fungal DNA, Mitochondrial Macromolecular Substances RNA, Fungal RNA, Messenger DNA Electron Transport Complex IV Endonucleases Single-Strand Specific DNA and RNA Endonucleases Adenosine Triphosphatases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Osinga K A
De Vries E
Van der Horst G
Tabak H F
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44 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-04-00
Pages
829-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557434
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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