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PMID: 7322920 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RNA synthesis in isolated yeast mitochondria.

Nucleic acids research ·Vol. 9 ·No. 23 ·1981-12-11 ·Pages 6369-77

Groot GS, van Harten-Loosbroek N, van Ommen GJ, Pijst HL

Abstract

Isolated yeast mitochondria incorporate added UTP into RNA. Amongst the products formed are the two rRNAs, 4S RNA and several components presumed to be mRNAs. In omega+ strains (containing an intervening sequence in the 21S rRNA gene) besides mature 21S rRNA a transcript could be detected still containing nucleotide sequences transcribed from this intervening sequence. In omega- strains (not containing this intervening sequence) also a longer form of the 21S rRNA could be observed. These results suggest that isolated yeast mitochondria are capable of carrying out RNA synthesis and processing, including splicing.

MeSH Terms
DNA, Mitochondrial/genetics Dactinomycin/pharmacology Hydrogen-Ion Concentration Kinetics Mitochondria/metabolism Nucleic Acid Hybridization RNA, Fungal/biosynthesis,genetics Saccharomyces/metabolism Transcription, Genetic/drug effects
Chemicals
DNA, Mitochondrial RNA, Fungal Dactinomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Groot G S
van Harten-Loosbroek N
van Ommen G J
Pijst H L
References (15)
15 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.23.6369
Published
1981-12-11
Pages
6369-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327609
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.23.6369 PMC Full Text View journal details
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