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PMID: 6328026 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phosphorylation of the ribosomal protein S6 is elevated in cells transformed by a variety of tumor viruses.

Journal of virology ·Vol. 50 ·No. 3 ·1984-06-00 ·Pages 966-9

Blenis J, Erikson RL

Abstract

We have analyzed the phosphorylation of the ribosomal protein S6 in several cultures of normal cells and cells transformed with RNA or DNA tumor viruses. Serum deprivation of confluent, normal cells results in low levels of S6 phosphorylation. In contrast, under identical conditions, this protein is highly phosphorylated in virally transformed cells. No differences in the phosphopeptides generated by one-dimensional limited proteolysis were detected in S6 prepared from serum-stimulated or transformed cells. These results suggest that a common event in oncogenic transformation is the phosphorylation of S6.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Cell Transformation, Neoplastic Chick Embryo Molecular Weight Phosphopeptides/analysis Phosphorylation Ribosomal Protein S6 Ribosomal Proteins/isolation & purification,metabolism Species Specificity
Chemicals
Phosphopeptides Ribosomal Protein S6 Ribosomal Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Blenis J
Erikson R L
References (29)
29 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.3.966-969.1984
Published
1984-06-00
Pages
966-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255770
Subset
IM
Grants
NCI NIH HHS · CA34943 · United States
External Links
PubMed source DOI: 10.1128/JVI.50.3.966-969.1984 PMC Full Text View journal details
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