Abstract
A complete nucleotide sequence of two ras-related yeast genes (c- rassc -1 and c- rassc -2) isolated from the yeast strain Saccharomyces cerevisiae is reported. They encode predicted polypeptides of 40,000 and 41,000 daltons, respectively. The N-terminal 170 amino acids from both genes show extensive amino acid homology to other ras genes from vertebrates, whereas their C-termini have diverged. These genes should be useful in the elucidation of a normal biological function of ras-related genes in a simple system like yeast.
MeSH Terms
Amino Acid Sequence
Animals
Base Sequence
DNA, Fungal/genetics
Fungal Proteins/genetics
Genes, Fungal
Mice
Molecular Weight
Oncogenes
Saccharomyces cerevisiae/genetics
Sarcoma Viruses, Murine/genetics
Species Specificity
Chemicals
DNA, Fungal
Fungal Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dhar R
Nieto A
Koller R
DeFeo-Jones D
Scolnick E M
References (16)
16 references, click to expand
-
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4
PMID: 265521
-
Saccharomyces cerevisiae synthesizes proteins related to the p21 gene product of ras genes found in mammals.
Mol Cell Biol. 1984 Jan;4(1):23-9
PMID: 6422257
-
Proposal for naming host cell-derived inserts in retrovirus genomes.
J Virol. 1981 Dec;40(3):953-7
PMID: 7321107
-
Identification of a precursor in the biosynthesis of the p21 transforming protein of harvey murine sarcoma virus.
J Virol. 1982 Apr;42(1):253-61
PMID: 6283143
-
Characterization of the phosphorylation sites and the surrounding amino acid sequences of the p21 transforming proteins coded for by the Harvey and Kirsten strains of murine sarcoma viruses.
J Biol Chem. 1982 Oct 10;257(19):11767-73
PMID: 6288698
-
Mechanism of activation of a human oncogene.
Nature. 1982 Nov 11;300(5888):143-9
PMID: 6290897
-
A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.
Nature. 1982 Nov 11;300(5888):149-52
PMID: 7133135
-
Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts.
Cell. 1983 Jun;33(2):519-27
PMID: 6305513
-
Transcription terminates in yeast distal to a control sequence.
Cell. 1983 Jun;33(2):607-14
PMID: 6305514
-
Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene.
Nature. 1983 Jun 30;303(5920):775-9
PMID: 6866079
-
Structure of the Ki-ras gene of the human lung carcinoma cell line Calu-1.
Nature. 1983 Aug 11-17;304(5926):497-500
PMID: 6308465
-
Structure and organization of the human Ki-ras proto-oncogene and a related processed pseudogene.
Nature. 1983 Aug 11-17;304(5926):501-6
PMID: 6308466
-
Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations.
Nature. 1983 Dec 15-21;306(5944):658-61
PMID: 6318112
-
A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product.
Nature. 1983 Dec 15-21;306(5944):704-7
PMID: 6318115
-
ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae.
Nature. 1983 Dec 15-21;306(5944):707-9
PMID: 6318116
-
Guanine nucleotide-binding activity as an assay for src protein of rat-derived murine sarcoma viruses.
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5355-9
PMID: 228288