Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli.

Nucleic acids research ·Vol. 12 ·No. 9 ·1984-05-11 ·Pages 3791-806

Boss MA, Kenten JH, Wood CR, Emtage JS

Abstract

Genes for a murine mu heavy chain and a lambda light chain immunoglobulin have been inserted into bacterial expression plasmids containing the Escherichia coli trp promoter and ribosome binding site. Induction of transcription from the trp promoter results in accumulation of both light and heavy chain polypeptides in appropriate host strains. Both proteins were found as insoluble products. Following extraction and purification of the immunoglobulin containing fractions, antigen binding activity was recovered. The activity demonstrates essentially the same properties as the antibody from the hybridoma from which the genes were cloned.

MeSH Terms

Animals Antibodies/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Genes Haptens Immunoglobulin Heavy Chains/genetics Immunoglobulin Light Chains/genetics Immunoglobulin lambda-Chains/genetics,isolation & purification Immunoglobulin mu-Chains/genetics,isolation & purification Kinetics Mice Plasmids

Chemicals

Antibodies Haptens Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin lambda-Chains Immunoglobulin mu-Chains DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Boss M A

Kenten J H

Wood C R

Emtage J S

References (19)

19 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.9.3791

Published

1984-05-11

Pages

3791-806

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318790

Subset

External Links

PubMed source DOI: 10.1093/nar/12.9.3791 PMC Full Text View journal details

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Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli.

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