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PMID: 6329736 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The identification and high level expression of a protein encoded by the yeast Ty element.

The EMBO journal ·Vol. 3 ·No. 5 ·1984-05-00 ·Pages 1115-9

Dobson MJ, Mellor J, Fulton AM, Roberts NA, Bowen BA, Kingsman SM, Kingsman AJ

Abstract

Transcription of the yeast Ty element, Ty1 -15, was placed under the control of the efficient PGK promoter on the yeast expression vector, pMA91 . In extracts of yeast transformants containing these constructions a new 52 K basic polypeptide was detected by one- and two-dimensional electrophoresis and was shown, by hybrid arrested translation, to be specifically encoded by the Ty element. The protein coding region was mapped to the first 1.45 kb of the transcribed region of Ty1 -15. These data show for the first time that Ty elements encode proteins and illustrate the general usefulness of high efficiency expression vectors for the detection of rare products.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/genetics Fungal Proteins/genetics Genetic Vectors Nucleic Acid Hybridization Plasmids Protein Biosynthesis Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA Transposable Elements Fungal Proteins DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Dobson M J
Mellor J
Fulton A M
Roberts N A
Bowen B A
Kingsman S M
Kingsman A J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-05-00
Pages
1115-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557482
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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