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PMID: 6329739 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A close association between sites of DNase I hypersensitivity and sites of enhanced cleavage by micrococcal nuclease in the 5'-flanking region of the actively transcribed ovalbumin gene.

The EMBO journal ·Vol. 3 ·No. 5 ·1984-05-00 ·Pages 1137-44

Kaye JS, Bellard M, Dretzen G, Bellard F, Chambon P

Abstract

The organization of chromatin was analysed in a segment of the chicken ovalbumin gene extending 6.5 kb upstream from the start site of transcription. Nuclei of chicken oviduct cells and of erythrocytes, and preparations of 'naked' DNA were digested with DNase I and with micrococcal nuclease. The locations of specific nuclease cleavage sites were determined by analyzing the fragments obtained with an indirect end-labeling technique. In oviduct nuclei there are four regions of DNase I hypersensitivity centered at approximately 0.15, 0.80, 3.2 and 6.0 kb upstream from the mRNA cap site. DNase I hypersensitive regions are absent from the 5'-flanking regions of erythrocyte nuclei. Micrococcal nuclease cleavage sites were found that are unique to oviduct nuclei and others that are enhanced in oviduct nuclei, relative to erythrocyte nuclei and to naked DNA. The locations of these micrococcal nuclease cleavage sites are closely associated with the DNase I hypersensitive regions. Nuclease hypersensitivity in the 5'-flanking region of oviduct nuclei reflects alterations in chromatin structure that are specifically correlated with gene expression. Our results suggest the presence at hypersensitive regions of specific proteins which alter the chromatin structure, making the DNA more accessible to nuclease attack.

MeSH Terms
Animals Base Composition Cell Nucleus/metabolism Chickens DNA Restriction Enzymes Deoxyribonuclease I Endodeoxyribonucleases/metabolism Erythrocytes/metabolism Female Genes Genetic Linkage Micrococcal Nuclease/metabolism Ovalbumin/genetics Oviducts/metabolism Transcription, Genetic
Chemicals
Ovalbumin Endodeoxyribonucleases DNA Restriction Enzymes Deoxyribonuclease I Micrococcal Nuclease
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kaye J S
Bellard M
Dretzen G
Bellard F
Chambon P
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59 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-05-00
Pages
1137-44
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557486
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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