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PMID: 6287416 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Differences in the nuclease sensitivity between the two alleles of the immunoglobulin kappa light chain genes in mouse liver and myeloma nuclei.

Nucleic acids research ·Vol. 10 ·No. 12 ·1982-06-25 ·Pages 3627-45

Weischet WO, Glotov BO, Schnell H, Zachau HG

Abstract

In mouse myeloma T the productive kappa light chain gene differs from its aberrantly rearranged allele in the patterns of DNAase I hypersensitive sites. In the region of the alleles where they are identical in sequence they have one site in common which lies 0.8 kb downstream of the coding region; but two sites upstream of and within the C gene segment (2) are found only on the non-productive allele. Within the region of different sequences both alleles have analogously located DNAase I hypersensitive sites; they lie 0.15 kb upstream of the respective leader segments and cover putative promoter sequences. Only one of the six DNAase I hypersensitive sites is also very sensitive towards micrococcal nuclease due to its particular DNA sequence. The non-rearranged gene studied in liver nuclei has no DNAase I hypersensitive sites but is preferentially cleaved in A/T rich regions.

MeSH Terms
Alleles Animals Cell Nucleus/metabolism DNA Restriction Enzymes Deoxyribonuclease I Deoxyribonucleases/metabolism Endonucleases/metabolism Genes Immunoglobulin Light Chains/genetics Immunoglobulin kappa-Chains/genetics Liver/immunology Mice Mice, Inbred BALB C Neoplasms, Experimental/immunology Plasmacytoma/immunology
Chemicals
Immunoglobulin Light Chains Immunoglobulin kappa-Chains Deoxyribonucleases Endonucleases DNA Restriction Enzymes Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Weischet W O
Glotov B O
Schnell H
Zachau H G
References (31)
31 references, click to expand
  1. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
    Biochem J. 1967 Jan;102(1):251-7 PMID: 5339944
  2. Transcription of the unrearranged mouse C kappa locus: sequence of the initiation region and comparison of activity with a rearranged V kappa-C kappa gene.
    Cell. 1981 Dec;27(3 Pt 2):593-602 PMID: 6101210
  3. Studies on bovine pancreatic deoxyribonuclease A. II. The effect of different bivalent metals on the specificity of degradation of DNA.
    Biochim Biophys Acta. 1973 Jun 8;312(1):85-102 PMID: 4353710
  4. Analysis of repeating DNA sequences by reassociation.
    Methods Enzymol. 1974;29:363-418 PMID: 4850571
  5. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  6. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  7. The effects of salt concentration and H-1 depletion on the digestion of calf thymus chromatin by micrococcal nuclease.
    Nucleic Acids Res. 1979;6(5):1843-62 PMID: 450715
  8. The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence.
    Cell. 1979 Apr;16(4):797-806 PMID: 455449
  9. Sequences at the somatic recombination sites of immunoglobulin light-chain genes.
    Nature. 1979 Jul 26;280(5720):288-94 PMID: 111144
  10. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 PMID: 115000
  11. Non-random cleavage of SV40 DNA in the compact minichromosome and free in solution by micrococcal nuclease.
    Biochem Biophys Res Commun. 1980 Jan 29;92(2):532-9 PMID: 6243943
  12. Nucleosome structure.
    Annu Rev Biochem. 1980;49:1115-56 PMID: 6996562
  13. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
    Nature. 1980 Aug 28;286(5776):854-60 PMID: 6774262
  14. Functional and non-functional joining in immunoglobulin light chain genes of a mouse myeloma.
    Nature. 1980 Oct 16;287(5783):603-7 PMID: 6776411
  15. Two rearranged immunoglobulin kappa light chain genes in one mouse myeloma.
    Nucleic Acids Res. 1980 Apr 25;8(8):1693-707 PMID: 6253943
  16. A rearranged DNA sequence possibly related to the translocation of immunoglobulin gene segments.
    Nucleic Acids Res. 1980 Apr 25;8(8):1709-20 PMID: 6253944
  17. Cloning of V region fragments from mouse liver DNA and localization of repetitive DNA sequences in the vicinity of immunoglobulin gene segments.
    Nucleic Acids Res. 1980 Apr 25;8(8):1721-9 PMID: 6253945
  18. Accessibility of expressed and non-expressed genes to a restriction nuclease.
    Nucleic Acids Res. 1980 Oct 24;8(20):4621-38 PMID: 6255426
  19. Rearranged and germline immunoglobulin kappa genes: different states of DNase I sensitivity of constant kappa genes in immunocompetent and nonimmune cells.
    Biochemistry. 1981 Feb 17;20(4):990-6 PMID: 6260146
  20. Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7102-6 PMID: 6938957
  21. The nucleotide sequence of a 5.5-kilobase DNA segment containing the mouse kappa immunoglobulin J and C region genes.
    J Biol Chem. 1981 May 25;256(10):5116-20 PMID: 6262318
  22. DNA sequence of the constant gene region of the mouse immunoglobulin kappa chain.
    Nucleic Acids Res. 1981 Feb 25;9(4):971-81 PMID: 6785724
  23. Allelic exclusion and nonproductive immunoglobulin gene rearrangements.
    Cell. 1981 Apr;24(1):1-3 PMID: 6972259
  24. Differences between germ-line and rearranged immunoglobulin V kappa coding sequences suggest a localized mutation mechanism.
    Nature. 1981 Jun 25;291(5817):668-70 PMID: 6264318
  25. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  26. Sequence specific cleavage of DNA by micrococcal nuclease.
    Nucleic Acids Res. 1981 Jun 25;9(12):2643-58 PMID: 7279658
  27. High sequence specificity of micrococcal nuclease.
    Nucleic Acids Res. 1981 Jun 25;9(12):2659-73 PMID: 6269057
  28. A 200 base pair region at the 5' end of the chicken adult beta-globin gene is accessible to nuclease digestion.
    Cell. 1981 Nov;27(1 Pt 2):45-55 PMID: 6276024
  29. DNAase I-hypersensitive sites of chromatin.
    Cell. 1981 Dec;27(3 Pt 2):413-5 PMID: 6101195
  30. Nucleosome arrangement on tRNA genes of Xenopus laevis.
    Cell. 1981 Dec;27(3 Pt 2):459-66 PMID: 6101200
  31. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.
    Biochem Biophys Res Commun. 1973 May 15;52(2):504-10 PMID: 4711166
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.12.3627
Published
1982-06-25
Pages
3627-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320740
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.12.3627 PMC Full Text View journal details
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