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PMID: 6785724 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA sequence of the constant gene region of the mouse immunoglobulin kappa chain.

Nucleic acids research ·Vol. 9 ·No. 4 ·1981-02-25 ·Pages 971-81

Altenburger W, Neumaier PS, Steinmetz M, Zachau HG

Abstract

The constant (C; ref. 3) gene segment of the immunoglobulin kappa light chain and about 1 kb upstream as well as downstream of the segment have been sequenced. The sequences of the C gene segment itself and parts of the upstream region were determined both in liver and in myeloma T DNA clones derived from the same mouse inbred strain. The sequences were identical, i.e. no somatic mutations were detected. Two sites in the region not coding for protein are discussed as possible targets of aberrant variable (V) gene translocations. Doublet frequencies were calculated in the approx. 2500 bp of the C region sequence reported in this paper and in the approx. 3400 bp of two rearranged V gene regions.

MeSH Terms
Animals Base Sequence Clone Cells/analysis DNA/analysis Gene Frequency Immunoglobulin Constant Regions/analysis Immunoglobulin Light Chains/analysis Immunoglobulin kappa-Chains/analysis Immunoglobulins/analysis Liver/analysis Mice
Chemicals
Immunoglobulin Constant Regions Immunoglobulin Light Chains Immunoglobulin kappa-Chains Immunoglobulins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Altenburger W
Neumaier P S
Steinmetz M
Zachau H G
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.4.971
Published
1981-02-25
Pages
971-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326726
Subset
IM
Databases
GENBANK
J00549, J00551, J00557, J00558, J00559, L00089, X00268
External Links
PubMed source DOI: 10.1093/nar/9.4.971 PMC Full Text View journal details
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