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PMID: 6330039 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of nitrate reductase genes from the cyanobacterium Anacystis nidulans.

Journal of bacteriology ·Vol. 159 ·No. 1 ·1984-07-00 ·Pages 36-41

Kuhlemeier CJ, Logtenberg T, Stoorvogel W, van Heugten HA, Borrias WE, van Arkel GA

Abstract

Anacystis nidulans, a non-nitrogen-fixing cyanobacterium, can fulfill its nitrogen requirement by the assimilation of nitrate. The first step in the pathway, the reduction of nitrate to nitrite, is catalyzed by the molybdo-protein nitrate reductase. In this study, newly developed techniques for gene cloning in A. nidulans R2 were used for the isolation of two genes involved in nitrate reduction. One gene was cloned by complementation of the corresponding mutant; the other gene was picked up from a cosmid gene library by using a restriction fragment containing the transposon-inactivated gene as a probe. Both genes were unlinked single-copy chromosomal genes. Transformation studies provided evidence for the existence of a third locus involved in nitrate reduction.

MeSH Terms
Cloning, Molecular Cyanobacteria/enzymology,genetics DNA Restriction Enzymes DNA Transposable Elements Genes Methylnitronitrosoguanidine/toxicity Mutation Nitrate Reductase (NADPH) Nitrate Reductases/genetics Nucleic Acid Hybridization Plasmids
Chemicals
DNA Transposable Elements Methylnitronitrosoguanidine Nitrate Reductases Nitrate Reductase (NADPH) DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kuhlemeier C J
Logtenberg T
Stoorvogel W
van Heugten H A
Borrias W E
van Arkel G A
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27 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.159.1.36-41.1984
Published
1984-07-00
Pages
36-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215588
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.159.1.36-41.1984 PMC Full Text View journal details
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