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PMID: 6276694 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Vectors for cloning in cyanobacteria: construction and characterization of two recombinant plasmids capable of transformation of Escherichia coli K12 and Anacystis nidulans R2.

Molecular & general genetics : MGG ·Vol. 184 ·No. 2 ·1981-00-00 ·Pages 249-54

Kuhlemeier CJ, Borrias WE, van den Hondel CA, van Arkel GA

Abstract

Two plasmids were constructed consisting of the E. coli vector pACYC184 and the cyanobacterial plasmid pUC1. These recombinants, designated pUC104 and pUC105, can be transformed to E. coli K12 as well as to the cyanobacterium Anacystis nidulans R2 and in both hosts they express their antibiotic markers. pUC104 and pUC105 differ with respect to the location and the orientation of the pACYC184 segment in pUC1. pUC104 was found to be stable under all circumstances. Transformation of pUC105 to A. nidulans R2 gave intact plasmids when chloramphenicol was the selective agent, but upon ampicillin selection a deletion derivative was produced identical to pUC1. Further characteristics of pUC104 and pUC105 are described and their usefulness as cloning vectors is discussed.

MeSH Terms
Chloramphenicol/pharmacology Cyanobacteria/genetics Escherichia coli/genetics Genetic Vectors Phenotype Plasmids R Factors Recombination, Genetic Transformation, Genetic
Chemicals
Chloramphenicol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kuhlemeier C J
Borrias W E
van den Hondel C A
van Arkel G A
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25 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00272912
Published
1981-00-00
Pages
249-54
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00272912
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