Translocation of an ampicillin resistance determinant within an R-factor aggregate in Salmonella panama.

Antonie van Leeuwenhoek ·Vol. 44 ·No. 2 ·1978-00-00 ·Pages 203-18

van Embden JD

Abstract

The molecular properties of the plasmids of a natural isolate of Salmonella panama have been studied. This strain, Sp477, harbours 5 different plasmids: the conjugative plasmid pRI477TF (molecular weight 20 megadaltons), the two non-conjugative plasmids, pRI477A and pRI477S, coding for ampicillin and streptomycin plus sulfonamide resistance respectively (molecular weights of both 5.6 megadaltons) and two cryptic plasmids with molecular weights of 1.0 and 2.7, megadaltons respectively. After conjugal transfer to Escherichia coli the ampicillin resistance determinant was frequently found to be integrated into pRI477TF or pRI477S. The translocatable sequence on pRI477A, designated as Tn901, resembles the TnA sublcass transposon TnA(1).

MeSH Terms

Ampicillin/pharmacology Conjugation, Genetic DNA, Bacterial/analysis DNA, Circular/analysis Molecular Weight Penicillin Resistance R Factors Recombination, Genetic Salmonella/analysis,drug effects,genetics

Chemicals

DNA, Bacterial DNA, Circular Ampicillin

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

van Embden J D

References (28)

28 references, click to expand

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Article Info

Journal

Antonie van Leeuwenhoek

Abbr.

Antonie Van Leeuwenhoek

ISSN

0003-6072

DOI

10.1007/BF00643223

Published

1978-00-00

Pages

203-18

Language

English

Region

Netherlands

NLM ID

0372625

Subset

External Links

PubMed source DOI: 10.1007/BF00643223 View journal details

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Translocation of an ampicillin resistance determinant within an R-factor aggregate in Salmonella panama.

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