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PMID: 6330682 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Primary structure of rabbit 18S ribosomal RNA determined by direct RNA sequence analysis.

Nucleic acids research ·Vol. 12 ·No. 11 ·1984-06-11 ·Pages 4731-45

Connaughton JF, Rairkar A, Lockard RE, Kumar A

Abstract

The primary structure of rabbit 18S ribosomal RNA was determined by nucleotide sequence analysis of the RNA directly. The rabbit rRNA was specifically cleaved with T1 ribonuclease, as well as with E. coli RNase H using a Pst 1 DNA linker to generate a specific set of overlapping fragments spanning the entire length of the molecule. Both intact and fragmented 18S rRNA were end-labeled with [32P], base-specifically cleaved enzymatically and chemically and nucleotide sequences determined from long polyacrylamide sequencing gels run in formamide. This approach permitted the detection of both cistron heterogeneities and modified bases. Specific nucleotide sequences within E. coli 16S rRNA previously implicated in polyribosome function, tRNA binding, and subunit association are also conserved within the rabbit 18S rRNA. This conservation suggests the likelihood that these regions have similar functions within the eukaryotic 40S subunit.

MeSH Terms
Animals Base Sequence Electrophoresis, Polyacrylamide Gel Endoribonucleases Escherichia coli/enzymology Phosphorus Radioisotopes Phylogeny RNA, Ribosomal Rabbits Rats Reticulocytes/analysis Ribonuclease H Ribonuclease T1 Saccharomyces cerevisiae/genetics Species Specificity Xenopus
Chemicals
Phosphorus Radioisotopes RNA, Ribosomal Endoribonucleases Ribonuclease H Ribonuclease T1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Connaughton J F
Rairkar A
Lockard R E
Kumar A
References (26)
26 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.11.4731
Published
1984-06-11
Pages
4731-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318871
Subset
IM
Grants
NIGMS NIH HHS · GM 28985 · United States
Databases
GENBANK
X00640
External Links
PubMed source DOI: 10.1093/nar/12.11.4731 PMC Full Text View journal details
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