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PMID: 6330687 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant.

Nucleic acids research ·Vol. 12 ·No. 12 ·1984-06-25 ·Pages 4849-63

Postle K, Nguyen TT, Bertrand KP

Abstract

The Tn10 tetR gene encodes the repressor that regulates transcription of the Tn10 tetracycline resistance determinant. We have determined the DNA sequence of the tetR gene and a 905 base pair region immediately 3' to tetR. The tetR gene is located on a 701 base pair HincII restriction fragment. Deletions at either end of this region eliminate synthesis of the wild-type TetR protein in E. coli minicells, and eliminate TetR activity as measured by repression of beta-galactosidase synthesis in tetA-lacZ operon fusion strains. Taken together, the DNA sequence and the genetic data indicate that tetR encodes a 207 amino acid protein with a calculated molecular weight of 23,328. This value is in good agreement with estimates of 23,000-25,000 based on electrophoretic mobility in SDS-polyacrylamide gels. There is 47% amino acid sequence homology between the deduced sequences of the Tn10 and RP1/Tn1721 TetR proteins. There is, in addition, significant amino acid sequence homology between an NH2-terminal region of the Tn10 TetR repressor and the DNA recognition regions of other DNA-binding proteins.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Restriction Enzymes DNA Transposable Elements Drug Resistance, Microbial Escherichia coli/genetics Genes Genes, Bacterial Plasmids Repressor Proteins/genetics Tetracycline/toxicity Transcription Factors/genetics Transcription, Genetic beta-Galactosidase/genetics
Chemicals
DNA Transposable Elements Repressor Proteins Transcription Factors DNA Restriction Enzymes beta-Galactosidase Tetracycline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Postle K
Nguyen T T
Bertrand K P
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54 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.12.4849
Published
1984-06-25
Pages
4849-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318884
Subset
IM
Grants
NIAID NIH HHS · AI00470 · United States
NIAID NIH HHS · AI16735 · United States
NIGMS NIH HHS · GM06702 · United States
Databases
GENBANK
J01830, K00615, K01493, X00694
External Links
PubMed source DOI: 10.1093/nar/12.12.4849 PMC Full Text View journal details
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