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PMID: 6333585 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Posttranscriptional modulation of gene expression in cultured rat hepatocytes.

Molecular and cellular biology ·Vol. 4 ·No. 9 ·1984-09-00 ·Pages 1929-34

Jefferson DM, Clayton DF, Darnell JE, Reid LM

Abstract

The maintenance of high levels of two liver-specific mRNAs in cultured hepatocytes was achieved in a serum-free hormonally defined cell culture medium. However, this maintenance of liver-specific mRNA levels did not correlate with the level of transcription of the genes but was apparently due to increased stabilization of the tissue-specific mRNAs. The mRNA stabilization did not occur in serum-supplemented medium. In both defined and serum-supplemented medium, actin and tubulin mRNAs were also greatly increased, in both cases predominantly if not entirely due to increased mRNA stability.

MeSH Terms
Actins/genetics Animals Cells, Cultured Culture Media Genes Kinetics Liver/metabolism Male Nucleic Acid Hybridization RNA Processing, Post-Transcriptional RNA, Messenger/genetics,isolation & purification Rats Rats, Inbred Strains Serum Albumin/genetics Transcription, Genetic Tubulin/genetics alpha 1-Antitrypsin/genetics
Chemicals
Actins Culture Media RNA, Messenger Serum Albumin Tubulin alpha 1-Antitrypsin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jefferson D M
Clayton D F
Darnell J E
Reid L M
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27 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.9.1929-1934.1984
Published
1984-09-00
Pages
1929-34
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369002
Subset
IM
Grants
NCI NIH HHS · CA16006-10 · United States
NCI NIH HHS · CA30117 · United States
NCI NIH HHS · CA33164 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.9.1929-1934.1984 PMC Full Text View journal details
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