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PMID: 6336328 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Splice junctions in adenovirus 2 early region 4 mRNAs: multiple splice sites produce 18 to 24 RNAs.

Journal of virology ·Vol. 50 ·No. 1 ·1984-04-00 ·Pages 106-17

Tigges MA, Raskas HJ

Abstract

We localized the splice junctions in adenovirus 2 early region 4 (E4) mRNAs. Processing of the E4 precursor RNA positioned the donor splice site of the 5' leader sequence adjacent to acceptor sites near the 5' ends of five of the six open reading regions in the E4 transcription unit. Of particular interest among the E4 mRNAs is an extensively spliced class which includes multiple species with sizes ranging from 1.1 to 0.75 kilobases (kb). Purified 1.1- to 0.75-kb mRNAs specified at least 10 polypeptides in vitro. We detected eight acceptor and two donor splice sites utilized in the deletion of the intron from the 3' portion of these mRNAs. E4 RNAs were isolated from the cytoplasm of infected cells at 5, 9, 12, and 18 h after infection. The E4 mRNAs were present throughout infection, but different members of the 1.1- to 0.7-kb class were predominant at each time assayed. Alternate splicing of the 3.0-kb E4 precursor RNA can generate as many as 25 mRNAs that encode at least 16 polypeptides.

MeSH Terms
Adenoviruses, Human/genetics,physiology Base Sequence Cell Line Genes, Viral Humans Nucleic Acid Hybridization RNA Splicing RNA, Messenger/analysis,genetics RNA, Viral/analysis,genetics Viral Proteins/genetics
Chemicals
RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tigges M A
Raskas H J
References (43)
43 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.1.106-117.1984
Published
1984-04-00
Pages
106-17
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255589
Subset
IM
Grants
NCI NIH HHS · NCI CA-16007 · United States
NCI NIH HHS · NCI CA-16217 · United States
Databases
GENBANK
J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946
External Links
PubMed source DOI: 10.1128/JVI.50.1.106-117.1984 PMC Full Text View journal details
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