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PMID: 6338896 Published · ppublish English Journal Article

The cellular content of non Hodgkin lymphomas: a comprehensive analysis using monoclonal antibodies and other surface marker techniques.

British journal of cancer ·Vol. 47 ·No. 3 ·1983-03-00 ·Pages 327-51

Habeshaw JA, Bailey D, Stansfeld AG, Greaves MF

Abstract

Five samples of tonsil, 10 reactive lymph nodes and 65 consecutive cases of non-Hodgkin lymphoma (NHL) were evaluated in suspension phenotyping with the monoclonal antibodies alpha Leu-I, alpha Leu-2a, alpha Leu-3a, OKT1, OKT3, OKT4, OKT6, OKT8, W6/32, 26/114, DA-2, 2DI, J5, AN51 and OKT9 together with conventional surface marking by rosetting (E, Fc gamma, Fc mu, C3b, C3d) and staining for surface and cytoplasmic immunoglobulin (SIg, CyIg) heavy and light chain classes. The results confirm the reproductability and specificity of staining with monoclonal antibodies against T cells and T cells subsets. Evidence is presented for reactivity of alpha Leu-I antibody with SIg positive and Ia positive cells in some lymphomas (centroblastic centrocytic, lymphocytic and immunoblastic), and 2 cases showed evidence of marking with OKT3 on SIg positive cells in T cell predominant immunoblastic lymphoma. Lymphoblastic lymphomas of T cell type expressed the marker OKT6. On the basis of these results criteria for the diagnosis of T cell lymphoma are suggested. The monoclonal antibody J5, reactive with C-ALL antigen, showed variable positivity, occasionally strong in B cells in cases of centroblastic and centrocytic follicular lymphoma. Proportions of cells staining with the monoclonal antibody OKT9 showed a correlation between levels of cellular expression of transferrin (trf) receptor and the histological grade of malignancy, OKT9+ cells being elevated in high grade lymphomas, and in some cases of transforming lymphoma of low grade histological class. These results are discussed and indicate the advantage of employing a wide range of defined monoclonal reagents in the phenotypic evaluation of NHL.

MeSH Terms
Adolescent Adult Aged Antibodies, Monoclonal/immunology Antibodies, Neoplasm/immunology Antigens, Surface/immunology B-Lymphocytes/immunology Child Child, Preschool Female Histocompatibility Antigens Class II/immunology Humans Immunoenzyme Techniques Lymph Nodes/immunology Lymphoid Tissue/immunology Lymphoma/immunology,pathology Lymphoma, Non-Hodgkin/immunology Male Middle Aged Palatine Tonsil/immunology Phenotype Receptors, Antigen, B-Cell/analysis Rosette Formation T-Lymphocytes/immunology
Chemicals
Antibodies, Monoclonal Antibodies, Neoplasm Antigens, Surface Histocompatibility Antigens Class II Receptors, Antigen, B-Cell
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Habeshaw J A
Bailey D
Stansfeld A G
Greaves M F
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1983.52
Published
1983-03-00
Pages
327-51
Language
English
Region
England
NLM ID
0370635
PMCID
PMC2011301
Subset
IM
External Links
PubMed source DOI: 10.1038/bjc.1983.52 PMC Full Text View journal details
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