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PMID: 6340118 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transcription termination in vitro at the tryptophan operon attenuator is controlled by secondary structures in the leader transcript.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 8 ·1983-04-00 ·Pages 2206-10

Stroynowski I, Kuroda M, Yanofsky C

Abstract

The role of alternative RNA secondary structures in regulating transcription termination at the attenuator of the tryptophan (trp) operon of Serratia marcescens was examined in vitro by transcribing mutant DNA templates having deletions of different segments of the trp leader region. Deletions that removed sequences corresponding to successive segments of postulated RNA secondary structures either increased or decreased transcription termination at the attenuator. The results obtained are consistent with the hypothesis that transcription termination results from RNA polymerase recognition of a particular RNA secondary structure, the terminator. This structure forms only in the absence of an alternative, preceding, RNA secondary structure, the antiterminator.

MeSH Terms
Chromosome Deletion Gene Expression Regulation Hydrogen Bonding Nucleic Acid Conformation Operon RNA, Messenger/genetics Serratia marcescens/genetics Structure-Activity Relationship Transcription, Genetic Tryptophan/genetics
Chemicals
RNA, Messenger Tryptophan
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stroynowski I
Kuroda M
Yanofsky C
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.8.2206
Published
1983-04-00
Pages
2206-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393787
Subset
IM
Grants
NIGMS NIH HHS · GM09738 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.8.2206 PMC Full Text View journal details
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