Home LiteratureArticle Details
PMID: 6154938 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Multivalent translational control of transcription termination at attenuator of ilvGEDA operon of Escherichia coli K-12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 4 ·1980-04-00 ·Pages 1862-6

Lawther RP, Hatfield GW

Abstract

The regulatory region for the ilvGEDA operon of Escherichia coli K-12 has been located and characterized. ilv leader RNA transcribed from this region is described, and the DNA sequence of the region is presented. This DNA sequence contains a transcription promoter, a region coding for a 32-amino-acid polypeptide containing multiple isoleucine, valine, and leucine codons, and a transcription termination site preceding the first structural gene. The mutually exclusive secondary structures of the leader RNA have been analyzed. On the basis of these data, a model for the multivalent attenuation of the ilvGEDA operon is proposed.

MeSH Terms
Amino Acids, Branched-Chain/genetics Base Sequence DNA, Bacterial/genetics Escherichia coli/genetics Nucleic Acid Conformation Operon Plasmids Protein Biosynthesis RNA, Bacterial/genetics RNA, Messenger/genetics Ribosomes/metabolism Transcription, Genetic
Chemicals
Amino Acids, Branched-Chain DNA, Bacterial RNA, Bacterial RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lawther R P
Hatfield G W
References (26)
26 references, click to expand
  1. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  2. Purification and characterization of AUA specific isoleucine transfer ribonucleic acid from Escherichia coli B.
    Biochemistry. 1974 Jan 15;13(2):300-7 PMID: 4589307
  3. Stability of ribonucleic acid double-stranded helices.
    J Mol Biol. 1974 Jul 15;86(4):843-53 PMID: 4427357
  4. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):784-8 PMID: 1093168
  5. New features of the regulation of the tryptophan operon.
    Science. 1975 Jul 4;189(4196):22-6 PMID: 1094538
  6. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  7. Initiation of in vitro mRNA synthesis from the wild-type lac promoter.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4394-8 PMID: 1060117
  8. Polarity and the regulation of the ilv gene cluster in Escherichia coli strain K-12.
    Mol Gen Genet. 1976 Oct 18;148(2):111-24 PMID: 790150
  9. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  10. Biochemical characterization of an Escherichia coli hisT strain.
    J Bacteriol. 1977 Apr;130(1):552-7 PMID: 192717
  11. Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4365-9 PMID: 337297
  12. Evidence for misreading during tRNA-dependent protein synthesis in vitro.
    J Biol Chem. 1978 May 25;253(10):3482-6 PMID: 348693
  13. Stuttering: high-level mistranslation in animal and bacterial cells.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1091-5 PMID: 349556
  14. Nucleotide sequence of the leader region of the phenylalanine operon of Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4271-5 PMID: 360214
  15. Nucleotide sequence of the attenuator region of the histidine operon of Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4276-80 PMID: 360215
  16. DNA sequence from the histidine operon control region: seven histidine codons in a row.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4281-5 PMID: 360216
  17. A computer aided oligonucleotide analysis provides a model sequence for RNA polymerase-promoter recognition in E.coli.
    Nucleic Acids Res. 1978 Oct;5(10):3759-73 PMID: 364417
  18. A site of action for tRNA mediated regulation of the ilvOEDA operon of Escherichia coli K12.
    Mol Gen Genet. 1978 Nov 29;167(2):227-34 PMID: 366386
  19. Single base-pair alterations in the Escherichia coli trp operon leader region that relieve transcription termination at the trp attenuator.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4833-7 PMID: 368800
  20. Mutations affecting the formation of acetohydroxy acid synthase II in Escherichia coli K-12.
    Mol Gen Genet. 1979 Feb 1;169(3):299-314 PMID: 372752
  21. Regulation of the threonine operon: tandem threonine and isoleucine codons in the control region and translational control of transcription termination.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1706-10 PMID: 287010
  22. leu operon of Salmonella typhimurium is controlled by an attenuation mechanism.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4941-5 PMID: 388423
  23. The nucleotide sequence preceding and including the beginning of the ilvE gene of the ilvGEDA operon of Escherichia coli K12.
    Nucleic Acids Res. 1979 Dec 20;7(8):2289-301 PMID: 392469
  24. Attenuation in the Escherichia coli tryptophan operon: role of RNA secondary structure involving the tryptophan codon region.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5524-8 PMID: 118451
  25. Alternative secondary structures of leader RNAs and the regulation of the trp, phe, his, thr, and leu operons.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6186-90 PMID: 392514
  26. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.4.1862
Published
1980-04-00
Pages
1862-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348608
Subset
IM
Databases
GENBANK
J01634, J01635, M10313, M17624
External Links
PubMed source DOI: 10.1073/pnas.77.4.1862 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2