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PMID: 6344021 Published · ppublish English Journal Article

Expression of hepatitis B virus surface antigen in yeast.

Nucleic acids research ·Vol. 11 ·No. 9 ·1983-05-11 ·Pages 2745-63

Hitzeman RA, Chen CY, Hagie FE, Patzer EJ, Liu CC, Estell DA, Miller JV, Yaffe A, Kleid DG, Levinson AD, Oppermann H

Abstract

The structural gene of Hepatitis B virus surface protein (HBsAg) was introduced into a plasmid capable of autonomous replication and selection in both the yeast Saccharomyces cerevisiae and E. coli. In this plasmid transcription of the HBsAg is initiated by the 5'-flanking sequence of the yeast 3-phosphoglycerate kinase (PGK) gene and terminated by the 3'-flanking region of the yeast TRP1 gene. Yeast cells containing this plasmid produce a new major species of mRNA of 1200 nucleotides in length coding for HBsAg. Viral surface antigen is made in nonglycosylated form at a level of about 1-2 percent of total yeast protein. A small fraction of this polypeptide (2-5 percent) is found in aggregated form upon yeast cell disruption by glass beads. This material is similar in size, density, and shape to the 22nm particle, isolated from the plasma of human hepatitis carriers, and induced comparable levels of HBsAg antibodies in mice when compared with the natural particle.

MeSH Terms
Cysteine/metabolism Gene Expression Regulation Hepatitis B Surface Antigens/genetics,immunology Methionine/metabolism Molecular Weight Saccharomyces cerevisiae/genetics,ultrastructure
Chemicals
Hepatitis B Surface Antigens Methionine Cysteine
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Hitzeman R A
Chen C Y
Hagie F E
Patzer E J
Liu C C
Estell D A
Miller J V
Yaffe A
Kleid D G
Levinson A D
Oppermann H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.9.2745
Published
1983-05-11
Pages
2745-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325921
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.9.2745 PMC Full Text View journal details
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