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PMID: 6933503 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 8 ·1980-08-00 ·Pages 4549-53

Dubois MF, Pourcel C, Rousset S, Chany C, Tiollais P

Abstract

A plasmid containing two cloned hepatitis B virus genomes in a tandem head-to-tail arrangement has been introduced into mouse fibroblasts by using cotransformation with the cloned herpes simplex virus thymidine kinase gene. Several copies of the plasmid were integrated into high molecular weight cellular DNA. The original tandem structure of the hepatitis B virus DNA was conserved. Hepatitis B surface antigen was synthesized by all the 15 clones examined. The other viral antigens were not detected. The surface antigen was excreted into the cell culture medium as particles having the same characteristics as those found in human serum. It is estimated that 2-4 X 10(4) particles were produced per mouse cell per 24 hr in two clones. This value corresponds to approximately 2-4 X 10(6) surface antigen polypeptides per cell per 24 hr.

MeSH Terms
Animals Cell Transformation, Viral Cells, Cultured DNA, Recombinant DNA, Viral/genetics Gene Expression Regulation Genes Genes, Viral Hepatitis B Surface Antigens/genetics Mice Transformation, Genetic
Chemicals
DNA, Recombinant DNA, Viral Hepatitis B Surface Antigens
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dubois M F
Pourcel C
Rousset S
Chany C
Tiollais P
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.8.4549
Published
1980-08-00
Pages
4549-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349881
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.8.4549 PMC Full Text View journal details
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