Replication of pBR322 DNA in stringent and relaxed strains of Escherichia coli.
Synthesis of both chromosomal and plasmid (pBR322) DNA was measured in E. coli strains differing in their relA allele (relA+:CP78; relA:CP79). It was found that upon limitation of a required amino acid or after valine treatment to trigger a stringent response synthesis of pBR322 DNA was stimulated only in the relaxed strain and was inhibited in its stringent counterpart. The results suggest that replication of plasmid DNA is negatively controlled by the relA+ allele.
12 references, click to expand
-
A rapid and economic method for estimation of the number of plasmid copies in Escherichia coli cells.Folia Microbiol (Praha). 1982;27(2):138-41 PMID: 7044920
-
Inititation and termination of chromosome replication in Escherichia coli subjected to amino acid starvation.J Bacteriol. 1980 Apr;142(1):236-42 PMID: 6246063
-
Two compounds implicated in the function of the RC gene of Escherichia coli.Nature. 1969 Mar 1;221(5183):838-41 PMID: 4885263
-
[Replication and expression of plasmid pBR 322 during discontinuous culture of a stringent and relaxed Escherichia coli strain].Z Allg Mikrobiol. 1983;23(6):367-74 PMID: 6356638
-
Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
-
The effect of alcohols on guanosine 5'-diphosphate-3'-diphosphate metabolism in stringent and relaxed Escherichia coli.J Biol Chem. 1980 Jul 10;255(13):6307-13 PMID: 6156159
-
Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.Proc Natl Acad Sci U S A. 1980 May;77(5):2450-4 PMID: 6156450
-
Stringent control in E. coli.Annu Rev Genet. 1979;13:393-415 PMID: 94252
-
An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate.J Chromatogr. 1969 Mar 11;40(1):103-9 PMID: 5777609
-
[Synthesis of poly(A)-containing RNA in outgrowing spores of Bacillus subtilis].Z Allg Mikrobiol. 1981;21(1):35-40 PMID: 6164168
-
Initiation and control of DNA synthesis.Annu Rev Biochem. 1969;38:569-604 PMID: 4979651
-
Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli.J Bacteriol. 1972 Jun;110(3):1135-46 PMID: 4555406
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
