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PMID: 6350235 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview.

The Histochemical journal ·Vol. 15 ·No. 7 ·1983-07-00 ·Pages 691-713

Ramaekers FC, Puts JJ, Moesker O, Kant A, Huysmans A, Haag D, Jap PH, Herman CJ, Vooijs GP

Abstract

Intermediate-sized filament proteins (IFP) are tissue specific in that antibodies to keratin, vimentin, desmin, glial fibrillary acidic protein (GFAP) and the neurofilament proteins can distinguish between cells of epithelial and mesenchymal origin as well as of myogenic and neural origin respectively. Malignant cells retain their tissue-specific IFP, which makes it possible to use these antibodies in tumour diagnosis. Carcinomas are exclusively detected by antibodies to keratin. Monoclonal antibodies to keratin have allowed the differentiation between subgroups of epithelial tumours until now between adenocarcinomas and squamous cell carcinomas. Lymphomas, melanomas and several soft tissue tumours are distinctly recognized by antibodies to vimentin. On the other hand, rhabdomyosarcomas and leiomyosarcomas are positive for desmin, while astrocytomas give a strong reaction with GFAP antibodies. Thus, antibodies to IFP are useful tools for differential diagnosis in surgical pathology.

MeSH Terms
Antibodies/immunology Diagnosis, Differential Histocytochemistry Humans Immunologic Techniques Intermediate Filament Proteins/immunology Neoplasms/diagnosis
Chemicals
Antibodies Intermediate Filament Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Ramaekers F C
Puts J J
Moesker O
Kant A
Huysmans A
Haag D
Jap P H
Herman C J
Vooijs G P
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Article Info
Journal
The Histochemical journal
Abbr.
Histochem J
ISSN
0018-2214
DOI
10.1007/BF01002988
Published
1983-07-00
Pages
691-713
Language
English
Region
Netherlands
NLM ID
0163161
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01002988
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