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PMID: 6350321 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Highly homologous filamin polypeptides have different distributions in avian slow and fast muscle fibers.

The Journal of cell biology ·Vol. 97 ·No. 3 ·1983-09-00 ·Pages 818-23

Gomer RH, Lazarides E

Abstract

The high molecular weight actin-binding protein filamin is located at the periphery of the Z disk in the fast adult chicken pectoral muscle (Gomer, R. H., and E. Lazarides, 1981, Cell, 23: 524-532). In contrast, we have found that in the slow anterior latissimus dorsi (ALD) muscle, filamin was additionally located throughout the l band as judged by immunofluorescence with affinity-purified antibodies on myofibrils and cryosections. The Z line proteins desmin and alpha-actinin, however, had the same distribution in ALD as they do in pectoral muscle. Quantitation of filamin and actin from the two muscle types showed that there was approximately 10 times as much filamin per actin in ALD myofibrils as in pectoral myofibrils. Filamin immunoprecipitated from ALD had an electrophoretic mobility in SDS polyacrylamide gels identical to that of pectoral myofibril filamin and slightly greater than that of chicken gizzard filamin. Two-dimensional peptide maps of filamin immunoprecipitated and labeled with 125I showed that ALD myofibril filamin was virtually identical to pectoral myofibril filamin and was distinct from chicken gizzard filamin.

MeSH Terms
Actinin/metabolism Actins/metabolism Animals Carrier Proteins/metabolism Chick Embryo Contractile Proteins/analysis,metabolism Desmin Filamins Fluorescent Antibody Technique Gelsolin Intermediate Filament Proteins/metabolism Microfilament Proteins Muscle Proteins/metabolism Muscles/analysis,metabolism Peptide Fragments/analysis
Chemicals
Actins Carrier Proteins Contractile Proteins Desmin Filamins Gelsolin Intermediate Filament Proteins Microfilament Proteins Muscle Proteins Peptide Fragments brevin Actinin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gomer R H
Lazarides E
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54 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.97.3.818
Published
1983-09-00
Pages
818-23
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112561
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.97.3.818 PMC Full Text View journal details
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