Home LiteratureArticle Details
PMID: 6360999 Published · ppublish English Comparative Study Journal Article

Identification of the structural gene and nonsense alleles for adenylate cyclase in Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 157 ·No. 1 ·1984-01-00 ·Pages 277-82

Matsumoto K, Uno I, Ishikawa T

Abstract

Tetraploid strains of Saccharomyces cerevisiae carrying different dosages of the CYR1+ gene have been constructed. Adenylate cyclase activity observed in these tetraploid strains was proportional to the dosage of the active CYR1+ gene. Of the 57 mutants requiring adenosine 3',5'-monophosphate for growth at 35 degrees C, two allelic temperature-sensitive cyr1 mutants produced thermolabile adenylate cyclase. Crude extract and plasma membrane fraction of cyr1 mutant cells had no adenylate cyclase activity when assayed with GTP or 5'-guanylyl imidodiphosphate in the presence of Mn2+ or Mg2+. Plasma membrane and Lubrol-soluble plasma membrane fractions obtained from the temperature-sensitive cyr1 mutant were thermolabile compared with those from the wild-type strain. Three cyr1 mutants carried nonsense mutations susceptible to ochre (UAA) suppressors, SUP3 and SUP-o, and had no detectable level of adenylate cyclase activity. It is concluded that the cyr1 mutants carry lesions in the structural gene for adenylate cyclase.

MeSH Terms
Adenylyl Cyclases/genetics Alleles Cell Membrane/enzymology Genes Genes, Fungal Mutation Polyploidy Saccharomyces cerevisiae/enzymology,genetics,isolation & purification Temperature
Chemicals
Adenylyl Cyclases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Matsumoto K
Uno I
Ishikawa T
References (21)
21 references, click to expand
  1. Properties and possible functions of the adenylate cyclase in plasma membranes of Saccharomyces cerevisiae.
    Mol Cell Biol. 1982 Dec;2(12):1481-91 PMID: 14582190
  2. Effect of gene dosage on tryptophan synthetase activity in Saccharomyces cerevisiae.
    Genetics. 1969 Mar;61(3):567-76 PMID: 5377464
  3. Nonsense mutations in the ade3 locus of Saccharomyces cerevisiae.
    Genetics. 1972 Jun;71(2):217-32 PMID: 4558325
  4. Suppressors in yeast.
    Curr Top Microbiol Immunol. 1974;64(0):1-47 PMID: 4602646
  5. Chitin synthetase zymogen is attached to the yeast plasma membrane.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3952-5 PMID: 1105572
  6. Adenosine 3':5'-cyclic monophosphate deficiency in Neurospora crassa.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):2995-9 PMID: 184453
  7. Interaction of super-repressible and dominant constitutive mutations for the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae.
    Mol Gen Genet. 1977 Apr 29;152(3):137-44 PMID: 327270
  8. Genetic control of galactokinase synthesis in Saccharomyces cerevisiae: evidence for constitutive expression of the positive regulatory gene gal4.
    J Bacteriol. 1978 May;134(2):446-57 PMID: 207666
  9. Reconstitution of hormone-sensitive adenylate cyclase activity with resolved components of the enzyme.
    J Biol Chem. 1978 Sep 25;253(18):6401-12 PMID: 210183
  10. Septum formation, cell division, and sporulation in mutants of yeast deficient in proteinase B.
    Proc Natl Acad Sci U S A. 1979 May;76(5):2395-9 PMID: 377296
  11. Function of positive regulatory gene gal4 in the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae: evidence that the GAL81 region codes for part of the gal4 protein.
    J Bacteriol. 1980 Feb;141(2):508-27 PMID: 6988385
  12. Yeast mating pheromone alpha factor inhibits adenylate cyclase.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1898-902 PMID: 6246513
  13. Biochemical properties of hormone-sensitive adenylate cyclase.
    Annu Rev Biochem. 1980;49:533-64 PMID: 6105841
  14. Mapping of the proteinase b structural gene PRB1, in Saccharomyces cerevisiae and identification of nonsense alleles within the locus.
    Genetics. 1980 Sep;96(1):137-46 PMID: 7009321
  15. Cyclic AMP may not be involved in catabolite repression in Saccharomyes cerevisiae: evidence from mutants capable of utilizing it as an adenine source.
    J Bacteriol. 1982 Apr;150(1):277-85 PMID: 6277865
  16. Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2355-9 PMID: 6285379
  17. Identification of the genetic locus for the structural gene and a new regulatory gene for the synthesis of repressible alkaline phosphatase in Saccharomyces cerevisiae.
    Mol Cell Biol. 1982 Feb;2(2):127-37 PMID: 7050668
  18. Characterization of cyclic AMP-requiring yeast mutants altered in the regulatory subunit of protein kinase.
    J Biol Chem. 1982 Dec 10;257(23):14110-5 PMID: 6292221
  19. Initiation of meiosis in yeast mutants defective in adenylate cyclase and cyclic AMP-dependent protein kinase.
    Cell. 1983 Feb;32(2):417-23 PMID: 6297794
  20. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  21. GENE DOSAGE AND GALACTOSE UTILIZATION BY SACCHAROMYCES TETRAPLOIDS.
    Genetics. 1963 Dec;48:1585-91 PMID: 14102801
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.157.1.277-282.1984
Published
1984-01-00
Pages
277-82
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215163
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.157.1.277-282.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2