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PMID: 6361499 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Missense and nonsense suppressors derived from a glycine tRNA by nucleotide insertion and deletion in vivo.

Molecular & general genetics : MGG ·Vol. 193 ·No. 1 ·1984-00-00 ·Pages 76-81

Murgola EJ, Prather NE, Pagel FT, Mims BH, Hijazi KA

Abstract

Beginning with a missense suppressor tRNA and a nonsense suppressor tRNA, both in Escherichia coli and each containing an extra nucleotide in the anticodon loop, we generated new suppressors in vivo by spontaneous deletion of specific nucleotides from the anticodon loop. In one experiment, the new suppressor was generated by a double mutational event, base substitution and nucleotide deletion. A novel ochre suppressor is also described. It is very efficient in nonsense suppression but has no ms2i6 modification of the A residue on the 3' side of the anticodon. The results have important implications for tRNA structure-function relationships, tRNA recognition by tRNA-modifying enzymes, mechanisms of deletion mutation, and tRNA evolution.

MeSH Terms
Anticodon/genetics Base Sequence Chromosome Deletion Chromosomes, Bacterial Escherichia coli/genetics Genotype Mutation Nucleic Acid Conformation RNA, Transfer, Amino Acyl/genetics Suppression, Genetic
Chemicals
Anticodon RNA, Transfer, Amino Acyl
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Murgola E J
Prather N E
Pagel F T
Mims B H
Hijazi K A
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24 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327417
Published
1984-00-00
Pages
76-81
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327417
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