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PMID: 6363387 Published · ppublish English Journal Article

Suppression of Escherichia coli recF mutations by recA-linked srfA mutations.

Journal of bacteriology ·Vol. 157 ·No. 2 ·1984-02-00 ·Pages 498-506

Volkert MR, Hartke MA

Abstract

Suppressors of recF (srfA) were found by selection for resistance to mitomycin C and UV irradiation in a recB21 recC22 sbcB15 recF143 strain. srfA mutations map in recA and are dominant to srfA+. They suppress both the DNA repair and the recombination deficiencies due to recF mutations. Therefore, RecA protein which is altered by the srfA mutation can allow genetic recombination to proceed in the absence of recB, recC, and recF functions. recF is also required for induction of the SOS response after UV damage. We propose that recF+ normally functions to allow the expression of two recA activities, one that is required for the RecF pathway of recombination and another that is required for SOS induction. The two RecA activities are different and are separable by mutation since srfA mutations permit recombination to proceed but have not caused a dramatic increase in SOS induction in recF mutants. According to this hypothesis, one role for recF in DNA repair and recombination is to modulate RecA activities to allow RecA to participate in these recF-dependent processes.

MeSH Terms
Escherichia coli/genetics Genes, Bacterial Genes, Dominant Genetic Linkage Mutation Operon Plasmids Rec A Recombinases/genetics Recombination, Genetic Suppression, Genetic
Chemicals
Rec A Recombinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Volkert M R
Hartke M A
References (35)
35 references, click to expand
  1. A model for three-point analysis of random general transduction.
    Genetics. 1966 Aug;54(2):405-10 PMID: 5338813
  2. Control of recA dependent activities in Escherichia coli: a possible role for the recF product.
    J Gen Microbiol. 1983 Mar;129(3):681-6 PMID: 6348206
  3. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  4. Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants.
    J Mol Biol. 1973 Oct 25;80(2):327-44 PMID: 4587405
  5. Thermal enhancement of ultraviolet mutability in a tif-1 uvrA derivative of Escherichia coli B-r: evidence that ultraviolet mutagenesis depends upon an inducible function.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1930-4 PMID: 4600265
  6. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 PMID: 4610576
  7. Progress toward a metabolic interpretation of genetic recombination of Escherichia coli and bacteriophage lambda.
    Genetics. 1974 Sep;78(1):259-71 PMID: 4613608
  8. Proteolytic cleavage of bacteriophage lambda repressor in induction.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):147-51 PMID: 1090931
  9. The beginning of an investigation of the role of recF in the pathways of metabolism of ultraviolet-irradiated DNA in Escherichia coli.
    Basic Life Sci. 1975;5A:283-91 PMID: 1103834
  10. The effect of lexA and recF mutations on post-replication repair and DNA synthesis in Escherichia coli K-12.
    Mol Gen Genet. 1975 Dec 1;141(3):189-205 PMID: 1107815
  11. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  12. Analysis of the role of recombination and repair in mutagenesis of Escherichia coli by UV irradiation.
    Genetics. 1977 Sep;87(1):1-18 PMID: 334628
  13. The dependence of postreplication repair on uvrB in a recF mutant of Escherichia coli K-12.
    Mol Gen Genet. 1977 Oct 24;155(3):279-86 PMID: 340884
  14. Influence of the recF143 mutation of Escherichia coli K12 on prophage lambda induction.
    Mutat Res. 1978 Oct;52(1):37-47 PMID: 366398
  15. W-reactivation of phage lambda in recF, recL, uvrA, and uvrB mutants of E. coli K-12.
    Mol Gen Genet. 1979 Feb 1;169(3):279-87 PMID: 372750
  16. A role for recF in repair of UV damage to DNA.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:887-92 PMID: 385232
  17. E. coli recA protein-directed cleavage of phage lambda repressor requires polynucleotide.
    Nature. 1980 Jan 3;283(5742):26-30 PMID: 6444245
  18. Deletions generated by the transposon Tn10 in the srl recA region of the Escherichia coli K-12 chromosome.
    Genetics. 1979 Oct;93(2):321-43 PMID: 395024
  19. Indirect and intragenic suppression of the lexA102 mutation in E. coli B/r.
    Mol Gen Genet. 1979;177(1):129-37 PMID: 395409
  20. Induction of prophage lambda without amplification of recA protein.
    Mol Gen Genet. 1980;178(2):317-23 PMID: 6446647
  21. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  22. Control of recA gene RNA in E. coli: regulatory and signal genes.
    Cell. 1980 Jul;20(3):731-7 PMID: 6998563
  23. Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12.
    J Bacteriol. 1981 Jan;145(1):521-32 PMID: 6257642
  24. Evidence that rnmB is the operator of the Escherichia coli recA gene.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1786-90 PMID: 7015345
  25. Physical mapping of the srl recA region of Escherichia coli: analysis of Tn10 generated insertions and deletions.
    Mol Gen Genet. 1981;183(3):497-504 PMID: 6278241
  26. Induction of E. coli recA protein via recBC and alternate pathways: quantitation by enzyme-linked immunosorbent assay (ELISA).
    Mol Gen Genet. 1982;185(2):275-82 PMID: 6283318
  27. Enzymatic activities of the RecA protein of Escherichia coli.
    Biochimie. 1982 Aug-Sep;64(8-9):611-6 PMID: 6128035
  28. recF-dependent recombination as a SOS function.
    Biochimie. 1982 Aug-Sep;64(8-9):629-32 PMID: 6215951
  29. recA operator mutations and their usefulness.
    Biochimie. 1982 Aug-Sep;64(8-9):669-75 PMID: 6291636
  30. Cell survival, UV-reactivation and induction of prophage lambda in Escherichia coli K12 overproducing RecA protein.
    Mol Gen Genet. 1982;188(1):37-43 PMID: 6217403
  31. Control of UV induction of recA protein.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):65-9 PMID: 6337375
  32. Genetic analysis of regulation of the RecF pathway of recombination in Escherichia coli K-12.
    J Bacteriol. 1983 Mar;153(3):1471-8 PMID: 6337999
  33. Effects of a recA operator mutation on mutant phenotypes conferred by lexA and recF mutations.
    Mutat Res. 1982 Nov;106(1):11-26 PMID: 6219286
  34. Inducible expression of a gene specific to the RecF pathway for recombination in Escherichia coli K12.
    Mol Gen Genet. 1983;190(1):162-7 PMID: 6343801
  35. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.
    J Bacteriol. 1969 Jan;97(1):244-9 PMID: 4884815
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.157.2.498-506.1984
Published
1984-02-00
Pages
498-506
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215275
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.157.2.498-506.1984 PMC Full Text View journal details
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