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PMID: 6363597 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monoclonal anti-Lyt-2.2 antibody blocks lectin-dependent cellular cytotoxicity of H-2-negative target cells.

The Journal of experimental medicine ·Vol. 159 ·No. 2 ·1984-02-01 ·Pages 551-8

Hünig T

Abstract

The hypothesis that blocking of cytotoxic T lymphocyte (CTL)-mediated cytolysis with anti-Lyt-2 antibodies acts at the level of inhibiting the interaction of the Lyt-2-bearing structure with H-2 class I molecules was tested. In agreement with the findings of others, purified anti-Lyt-2.2 inhibited both antigen-specific lysis and lectin-dependent cellular cytotoxicity (LDCC). LDCC of H-2-positive and H-2-negative target cells was similarly inhibited by this antibody. As expected, this effect was specific for CTL expressing the Lyt-2.2 allele, in contrast to blocking with a rat monoclonal antibody to the murine LFA-1 antigen. The implications of this finding for the function of the Lyt-2 antigen in CTL-target cell interaction are discussed.

MeSH Terms
Animals Antibodies, Monoclonal/physiology Antigens, Surface/immunology Binding, Competitive Cytotoxicity, Immunologic H-2 Antigens/immunology Isoantibodies/physiology Lectins/pharmacology Lymphocyte Activation Lymphocyte Function-Associated Antigen-1 Mice Mice, Inbred BALB C T-Lymphocytes, Cytotoxic/immunology
Chemicals
Antibodies, Monoclonal Antigens, Surface H-2 Antigens Isoantibodies Lectins Lymphocyte Function-Associated Antigen-1 Lyt antibodies
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Hünig T
References (20)
20 references, click to expand
  1. Regulation of cellular and humoral immune responses by T-cell subclasses.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:23-32 PMID: 302190
  2. The human T cell receptor: analysis with cytotoxic T cell clones.
    Immunol Rev. 1983;74:83-112 PMID: 6195086
  3. Helper cells activated by allogeneic H-2K or H-2D differences have a Ly phenotype distinct from those responsive to I differences.
    J Immunol. 1979 Feb;122(2):383-91 PMID: 310839
  4. Studies on the induction and expression of T cell-mediated immunity. X. Inhibition by Lyt 2,3 antisera of cytotoxic T lymphocyte-mediated antigen-specific and -nonspecific cytotoxicity: evidence for the blocking of the binding between T lymphocytes and target cells and not the post-binding cytolytic steps.
    J Immunol. 1980 Dec;125(6):2444-53 PMID: 6159409
  5. The Lyt phenotype of a long-term allospecific T cell line. Both helper and killer activities to IA are mediated by Ly-1 cells.
    Eur J Immunol. 1981 Mar;11(3):175-80 PMID: 6972304
  6. Monoclonal antibody to a novel lymphocyte function-associated antigen (LFA-1): mechanism of blockade of T lymphocyte-mediated killing and effects on other T and B lymphocyte functions.
    J Immunol. 1981 Aug;127(2):590-5 PMID: 6166678
  7. T lymphocyte-mediated cytolysis. II. Role of target cell histocompatibility antigens in recognition and lysis.
    J Immunol. 1981 Aug;127(2):782-7 PMID: 6265557
  8. Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity.
    J Exp Med. 1981 Jun 1;153(6):1503-16 PMID: 6166718
  9. Activation of T lymphocytes by lectins and carbohydrate-oxidizing reagents viewed as an immunological recognition of cell-surface modifications seen in the context of "self" major histocompatibility complex antigens.
    Eur J Immunol. 1981 Jun;11(6):475-83 PMID: 6266838
  10. Inhibition of murine T cell-mediated cytolysis and T cell proliferation by a rat monoclonal antibody immunoprecipitating two lymphoid cell surface polypeptides of 94 000 and 180 000 molecular weight.
    Eur J Immunol. 1982 Jan;12(1):60-9 PMID: 6977452
  11. Long-term human cytolytic T-cell lines allospecific for HLA-DR6 antigen are OKT4+.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2365-9 PMID: 6980419
  12. Cytotoxic T lymphocyte lines reactive against murine plasmacytoma antigens: dissociation of cytotoxicity and Lyt-2 expression.
    Eur J Immunol. 1982 Oct;12(10):831-7 PMID: 6184236
  13. Clonal heterogeneity in the functional requirement for Lyt-2/3 molecules on cytolytic T lymphocytes: analysis by antibody blocking and selective trypsinization.
    J Exp Med. 1982 Dec 1;156(6):1711-22 PMID: 6983559
  14. Possible involvement of the OKT4 molecule in T cell recognition of class II HLA antigens. Evidence from studies of cytotoxic T lymphocytes specific for SB antigens.
    J Exp Med. 1982 Oct 1;156(4):1065-76 PMID: 6984061
  15. Induction of cytolytic T lymphocytes by mitogenic lectins is specifically inhibited by anti-Lyt-2 antibodies.
    Eur J Immunol. 1983 Jun;13(6):476-80 PMID: 6602709
  16. Molecular interactions in T-cell-mediated cytotoxicity.
    Immunol Rev. 1983;72:119-41 PMID: 6192075
  17. The mechanism of CTL-mediated killing: monoclonal antibody analysis of the roles of killer and target-cell membrane proteins.
    Immunol Rev. 1983;72:73-96 PMID: 6347869
  18. T cell subsets and the recognition of MHC class.
    Immunol Rev. 1983;74:129-42 PMID: 6226585
  19. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity.
    Immunol Rev. 1983;74:29-56 PMID: 6195085
  20. H-2 antigens of the thymus determine lymphocyte specificity.
    J Exp Med. 1978 Sep 1;148(3):766-75 PMID: 308986
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.159.2.551
Published
1984-02-01
Pages
551-8
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187235
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.159.2.551 PMC Full Text View journal details
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