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PMID: 6369314 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Inducible repair of phosphotriesters in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 24 ·1983-12-00 ·Pages 7380-4

McCarthy JG, Edington BV, Schendel PF

Abstract

Extracts from Escherichia coli cells induced for the adaptive response have been prepared that are capable of repairing O6-methylguanine, O4-methylthymine, and the phosphotriesters produced on the DNA backbone by alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The phosphotriesters are repaired by a methyltransferase distinct from the one that demethylates O6-methylguanine. We propose that this increased capacity to repair phosphotriesters accounts for much of the increased resistance to MNNG toxicity seen in cultures induced for the adaptive response.

MeSH Terms
DNA Repair Deoxyribonucleotides/isolation & purification Escherichia coli/genetics Kinetics Methylation Methylnitronitrosoguanidine/pharmacology Methyltransferases/genetics,metabolism
Chemicals
Deoxyribonucleotides Methylnitronitrosoguanidine Methyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McCarthy J G
Edington B V
Schendel P F
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.24.7380
Published
1983-12-00
Pages
7380-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389954
Subset
IM
Grants
NCI NIH HHS · CA14733 · United States
NCI NIH HHS · CA32182 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.24.7380 PMC Full Text View journal details
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