Home LiteratureArticle Details
PMID: 6370241 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the C1q receptor on a human macrophage cell line, U937.

The Biochemical journal ·Vol. 218 ·No. 2 ·1984-03-01 ·Pages 547-55

Arvieux J, Reboul A, Bensa JC, Colomb MG

Abstract

The binding of C1q to the human macrophage cell line U937 has been studied. Fluorescence microscopy with fluorescein-conjugated F(ab')2 anti-C1q antibody showed that 100% of the cell population is able to bind exogenous C1q. Monomeric C1q binding to U937 cells is very weak at normal ionic strength (I0.15) and was therefore investigated at I0.07, conditions which stabilize the binding. However, aggregation of C1q on dextran sulphate or a lipid A-rich lipopolysaccharide allowed a firm, binding at I0.15. Quantitative binding studies with monomeric 125I-C1q showed a concentration-dependent, saturable, specific and reversible binding involving specific membrane receptors. Scatchard plots of C1q binding indicated [1.6 +/- 0.7 (1 S.D.)] X 10(6) sites per cell with an equilibrium constant of (2.9 +/- 1.8) X 10(7) M-1 at I0.07. The location of the molecule region mediating C1q binding was established with collagen-like fragments prepared by partial pepsin digestion, confirming earlier results obtained by inhibition studies.

MeSH Terms
Binding Sites Carrier Proteins Cell Line Collagen/metabolism Fluorescent Antibody Technique Humans Hyaluronan Receptors Kinetics Macrophages/immunology Membrane Glycoproteins Mitochondrial Proteins Osmolar Concentration Peptide Fragments/metabolism Peptide Hydrolases/pharmacology Receptors, Complement/drug effects,metabolism
Chemicals
C1QBP protein, human Carrier Proteins Hyaluronan Receptors Membrane Glycoproteins Mitochondrial Proteins Peptide Fragments Receptors, Complement complement 1q receptor Collagen Peptide Hydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Arvieux J
Reboul A
Bensa J C
Colomb M G
References (27)
27 references, click to expand
  1. A simple technique for the isolation of monoclonal IgG and IgA.
    Rev Eur Etud Clin Biol. 1970 Dec;15(10):1115-21 PMID: 5517893
  2. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  3. Effect of chemical and enzymatic radioiodination on in vitro human Clq activities.
    J Immunol. 1973 Mar;110(3):820-8 PMID: 4734666
  4. Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
    Int J Cancer. 1976 May 15;17(5):565-77 PMID: 178611
  5. Isolation, by partial pepsin digestion, of the three collagen-like regions present in subcomponent Clq of the first component of human complement.
    Biochem J. 1976 Apr 1;155(1):5-17 PMID: 7240
  6. Interactions among heparin, cold-insoluble globulin, and fibrinogen in formation of the heparin-precipitable fraction of plasma.
    J Clin Invest. 1977 Oct;60(4):855-65 PMID: 19499
  7. Lysis of C1Q-coated chicken erythrocytes by human lymphoblastoid cell lines.
    J Immunol. 1978 Jan;120(1):27-32 PMID: 564373
  8. The binding of human and guinea-pig IgG subclasses to homologous macrophage and monocyte Fc receptors.
    Immunology. 1978 Jul;35(1):115-23 PMID: 680795
  9. In vitro activation of a human macrophage-like cell line.
    Nature. 1979 May 24;279(5711):328-31 PMID: 450085
  10. Ingestion of dyed-opsonised yeasts as a simple way of detecting phagocytes in lymphocyte preparations. Cytophilic binding of immunoglobulins by ingesting cells.
    J Immunol Methods. 1979;27(2):175-87 PMID: 379233
  11. Differential elution of Clq, Clr and Cls from human Cl bound to immune aggregates. Use in the rapid purification of Cl subcomponents.
    Mol Immunol. 1979 Jul;16(7):445-50 PMID: 40870
  12. A rosette assay for the determination of C 1 q receptor-bearing cells.
    Eur J Immunol. 1979 Oct;9(10):797-801 PMID: 118036
  13. Analysis of receptor-mediated C1q binding to human peripheral blood mononuclear cells.
    J Immunol. 1980 Oct;125(4):1658-64 PMID: 7410850
  14. Characterization of the Fc receptor for IgG on a human macrophage cell line, U937.
    J Immunol. 1980 Dec;125(6):2735-41 PMID: 7000905
  15. Purified proenzyme C1r. Some characteristics of its activation and subsequent proteolytic cleavage.
    Biochim Biophys Acta. 1980 Nov 6;616(1):116-29 PMID: 6254570
  16. MLC-conditioned media stimulate an increase in Fc receptors on human macrophages.
    J Immunol. 1981 Feb;126(2):666-8 PMID: 6450248
  17. Identification of types of cells in human peripheral blood that bind C1q.
    J Immunol. 1981 Mar;126(3):1174-9 PMID: 6970220
  18. Evidence that C1q, a subcomponent of the first component of complement, is an Fc receptor of peritoneal and alveolar macrophages.
    Immunobiology. 1980 Apr;157(1):54-61 PMID: 7203513
  19. Immunofluorescence studies on the subcomponents of the first component of complement (C1): detection of C1q and C1s in different cells of biopsy material and on human as well as on guinea pig peritoneal macrophages.
    Immunobiology. 1981;158(3):213-24 PMID: 7011949
  20. Fluid phase activation of proenzymic C1r purified by affinity chromatography.
    Biochim Biophys Acta. 1982 Jan 4;700(1):118-26 PMID: 6275893
  21. Fibronectin interacts with Clq, a subcomponent of the first component of complement.
    Immunol Lett. 1982 Jan;4(1):39-43 PMID: 6277780
  22. Preparation and properties of human C1 inhibitor.
    Methods Enzymol. 1981;80 Pt C:43-54 PMID: 6281621
  23. Interaction of C1q with DNA.
    Mol Immunol. 1982 Sep;19(9):1179-87 PMID: 6983028
  24. Chemical studies on the isolated collagen-like and globular fragment of complement component C1q. Comparative studies on bovine and human C1q.
    Biochim Biophys Acta. 1983 Jan 12;742(1):122-8 PMID: 6600626
  25. Proteins involved in the activation and control of the two pathways of human complement.
    Biochem Soc Trans. 1983 Jan;11(1):1-12 PMID: 6298023
  26. Measurement of the association constants of the complexes formed between intact C1q or pepsin-treated C1q stalks and the unactivated or activated C1r2C1s2 tetramers.
    Mol Immunol. 1983 Jan;20(1):53-66 PMID: 6304502
  27. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.
    J Biol Chem. 1961 May;236:1372-9 PMID: 13767412
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2180547
Published
1984-03-01
Pages
547-55
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1153371
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2180547 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2