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PMID: 6370956 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Role of the nifQ gene product in the incorporation of molybdenum into nitrogenase in Klebsiella pneumoniae.

Journal of bacteriology ·Vol. 158 ·No. 1 ·1984-04-00 ·Pages 187-94

Imperial J, Ugalde RA, Shah VK, Brill WJ

Abstract

NifQ- mutants of Klebsiella pneumoniae are defective in nitrogen fixation due to an elevated requirement for molybdenum. When millimolar concentrations of molybdate were added to the medium, the effects of the nifQ mutations were suppressed. NifQ- mutants were not impaired in the uptake of molybdate, but molybdate accumulation was defective in these mutants. All of the nif-coded proteins were present in NifQ- cells derepressed in the absence of molybdenum. Molybdenum-activatable nitrogenase component I was found at the same level observed in the wild type. Molybdenum, thus, does not play a role in nif expression or in the short-term stability of nif-coded proteins. The defect in NifQ- mutants was in the incorporation of molybdenum into nitrogenase component I. The nifQ gene product acts together with the products of nifB, nifN, and nifE in the biosynthesis of the iron-molybdenum cofactor of nitrogenase.

MeSH Terms
Biological Transport Genes, Bacterial Klebsiella pneumoniae/enzymology,genetics Molybdenum/analysis,metabolism,pharmacology Nitrogen Fixation Nitrogenase/analysis,metabolism Operon
Chemicals
Molybdenum Nitrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Imperial J
Ugalde R A
Shah V K
Brill W J
References (30)
30 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.1.187-194.1984
Published
1984-04-00
Pages
187-94
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215397
Subset
IM
Grants
NIGMS NIH HHS · GM22130 · United States
External Links
PubMed source DOI: 10.1128/jb.158.1.187-194.1984 PMC Full Text View journal details
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