Electron transport to nitrogenase in Klebsiella pneumoniae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 5 ·1980-05-00 ·Pages 2555-8

Nieva-Gómez D, Roberts GP, Klevickis S, Brill WJ

Abstract

Cell-free extracts of nifF and nifJ mutants of Klebsiella pneumoniae are unable to couple acetylene reduction (N2 fixation) by nitrogenase to the oxidation of organic metabolites. However, nifF and nifJ mutants can complement each other in vitro to establish the coupling. This indicates that the products of the nifF and nifJ genes constitute essential elements of the physiological electron pathway to nitrogenase. The electron-transfer-active product of the nifF gene, a flavoprotein, has been purified.

MeSH Terms

Cell-Free System Electron Transport Genetic Complementation Test Klebsiella pneumoniae/enzymology,genetics,metabolism Mutation Nitrogenase/genetics,metabolism

Chemicals

Nitrogenase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Nieva-Gómez D

Roberts G P

Klevickis S

Brill W J

References (19)

19 references, click to expand

Electron transport carriers involved in nitrogen fixation by the coliform, Klebsiella pneumoniae.
J Gen Microbiol. 1974 Jul;83(0):153-64 PMID: 4153345
The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier.
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1079-86 PMID: 5264138
Nitrogenase V. The effect of Mo, W and V on the synthesis of nitrogenase components in Azotobacter vinelandii.
Biochim Biophys Acta. 1974 Aug 7;362(1):160-6 PMID: 4422774
Isolation and characteristics of flavodoxin from nitrogen-fixing Clostridium pasteurianum.
J Biol Chem. 1966 Jun 25;241(12):2752-6 PMID: 5912352
A photochemical procedure for reduction of oxidation-reduction proteins employing deazariboflavin as catalyst.
J Biol Chem. 1977 Aug 25;252(16):5612-4 PMID: 407227
Electron transport to nitrogenase in Azotobacter chroococcum: Azotobacter flavodoxin hydroquinone as an electron donor.
FEBS Lett. 1972 Oct 15;27(1):63-67 PMID: 11946808
The structure of the oxidized form of clostridial flavodoxin at 1.9-A resolution.
J Biol Chem. 1974 Jul 25;249(14):4383-92 PMID: 4843141
Construction of a P plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae.
Nature. 1976 Mar 18;260(5548):268-71 PMID: 768778
Nitrogenase. I. Repression and derepression of the iron-molybdenum and iron proteins of nitrogenase in Azotobacter vinelandii.
Biochim Biophys Acta. 1972 Feb 28;256(2):498-511 PMID: 5016550
The isolation and characterization of Rhodospirillum rubrum flavodoxin.
Biochem Biophys Res Commun. 1971 Oct 15;45(2):327-36 PMID: 4400790
Electron paramagnetic resonance of nitrogenase and nitrogenase components from Clostridium pasteurianum W5 and Azotobacter vinelandii OP.
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3142-5 PMID: 4343957
Roles of nifF and nifJ gene products in electron transport to nitrogenase in Klebsiella pneumoniae.
J Bacteriol. 1980 Feb;141(2):470-5 PMID: 6988383
Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2.
J Bacteriol. 1975 Mar;121(3):759-65 PMID: 1090602
Azotobacter free-radical flavoprotein. Preparation and properties of the apoprotein.
Biochemistry. 1968 Jul;7(7):2666-72 PMID: 5660081
A rapid micromethod for determination of FMN and FAD in mixtures.
Anal Biochem. 1973 May;53(1):332-6 PMID: 4145740
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
High resolution two-dimensional electrophoresis of proteins.
J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae.
J Bacteriol. 1978 Oct;136(1):267-79 PMID: 361694
Chemical study of two flavodoxins extracted from sulfate reducing bacteria.
Biochem Biophys Res Commun. 1970 Mar 12;38(5):965-72 PMID: 5437345

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.5.2555

Published

1980-05-00

Pages

2555-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC349440

Subset

External Links

PubMed source DOI: 10.1073/pnas.77.5.2555 PMC Full Text View journal details

Analysis Services

Electron transport to nitrogenase in Klebsiella pneumoniae.

Contact

WeChat Official Account