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PMID: 6374427 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulation of galactokinase (GAL1) enzyme accumulation in Saccharomyces cerevisiae.

Molecular and cellular biochemistry ·Vol. 61 ·No. 2 ·1984-00-00 ·Pages 173-82

Yarger JG, Halvorson HO, Hopper JE

Abstract

The regulation of GAL1 RNA and enzyme synthesis has been investigated in Saccharomyces cerevisiae. We have shown that the induction of GAL10 and GAL1 RNAs is coordinate. GAL1 RNA transcripts appear within 4.5 to 6 min and galactokinase synthesis within 6 to 9 min. Steady-state RNA levels were reached within 50 min and the steady-state rate of galactokinase enzyme synthesis within 40-50 min. From these kinetic studies, the initial induction of GAL1 enzyme activity is apparently under transcriptional control. In addition, during early induction, two galactokinase enzyme activities were detected; a major stable form and a minor unstable form.

MeSH Terms
Enzyme Induction Galactokinase/biosynthesis,genetics,metabolism Kinetics Nucleic Acid Hybridization Plants/metabolism Plasmids Protein Biosynthesis Saccharomyces cerevisiae/enzymology Transcription, Genetic Triticum/metabolism
Chemicals
Galactokinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yarger J G
Halvorson H O
Hopper J E
References (21)
21 references, click to expand
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Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF00222494
Published
1984-00-00
Pages
173-82
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
Grants
NIAID NIH HHS · AI 10610 · United States
NIGMS NIH HHS · GM 23717 · United States
External Links
PubMed source DOI: 10.1007/BF00222494 View journal details
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