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PMID: 6378886 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Positive control of expression of the argECBH gene cluster in vitro by guanosine 5'-diphosphate 3'-diphosphate.

Journal of bacteriology ·Vol. 159 ·No. 2 ·1984-08-00 ·Pages 647-51

Zidwick MJ, Korshus J, Rogers P

Abstract

By using a cell-free system derived from Escherichia coli, it was found that guanosine 5'-diphosphate 3'-diphosphate (ppGpp) was a positive effector for expression of both wings of bidirectionally transcribed argECBH gene cluster. A 7- to 20-fold increase in the synthesis of both argininosuccinase (the argH enzyme) and N-acetylornithinase (the argE enzyme) resulted with added ppGpp (0.2 mM optimum). Synthesis of hybridizable argECBH mRNA was enhanced only 30 to 100% by added ppGpp. Of the various guanosine nucleotides tested, only pppGpp mimicked ppGpp. Added ppGpp had no important effect upon (i) measurable argE or argH enzyme activity, (ii) total protein synthesis in the cell-free system, or (iii) the rate of decay of hybridizable argECBH mRNA. With extracts of an argR+ strain, added ppGpp had no effect on the repression of enzyme or mRNA synthesis by L-arginine. By using a two-stage system in which the bulk of argECBH mRNA was synthesized while protein synthesis was delayed, we showed that ppGpp acted at some point during transcription.

MeSH Terms
Escherichia coli/drug effects,enzymology,genetics Genes/drug effects Genes, Bacterial/drug effects Guanine Nucleotides/pharmacology Guanosine Tetraphosphate/pharmacology Kinetics Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics Transcription, Genetic/drug effects
Chemicals
Guanine Nucleotides RNA, Messenger Guanosine Tetraphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zidwick M J
Korshus J
Rogers P
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19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.159.2.647-651.1984
Published
1984-08-00
Pages
647-51
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215692
Subset
IM
Grants
NIGMS NIH HHS · GM-25320 · United States
External Links
PubMed source DOI: 10.1128/jb.159.2.647-651.1984 PMC Full Text View journal details
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