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PMID: 6396080 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structural features of yeast tRNA genes which affect transcription factor binding.

The EMBO journal ·Vol. 3 ·No. 12 ·1984-12-01 ·Pages 2793-800

Baker RE, Hall BD

Abstract

Transcription of yeast tRNA genes in vitro requires, in addition to RNA polymerase III, two accessory factors which are resolved by ion-exchange chromatography. One of these transcription factors (factor C) binds to tRNA genes. The stability of factor C-tRNA gene complexes is gene-dependent: the tRNAAGGArg gene forms a highly stable complex while tRNA3Leu and tRNATyr gene complexes are unstable under our standard assay conditions. To determine how differences in tRNA gene structure affect factor C binding, mutant tRNATyr genes, internally deleted tRNA3Leu genes and hybrid transcription units containing both tRNATyr and tRNA3Leu segments were compared in their abilities to stably bind factor C. Sequence changes in either of the two highly conserved promoter elements (A block and B block) affect factor C complex stability. Changes towards the consensus sequence increase complex stability while changes away from the consensus sequence drastically reduce stability. Also, the distance separating the A and B blocks affects complex stability; 34-53 bp gives highest stability. These results indicate that the stable binding of transcription factor C to tRNA genes involves interactions with both A block and B block sequences.

MeSH Terms

Base Sequence Genes, Fungal Kinetics Plasmids RNA, Transfer/genetics,metabolism Saccharomyces cerevisiae/genetics Temperature Transcription Factors/metabolism Transcription, Genetic

Chemicals

Transcription Factors RNA, Transfer

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Baker R E

Hall B D

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24 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1984-12-01

Pages

2793-800

Language

English

Region

England

NLM ID

8208664

PMCID

PMC557768

Subset

External Links

PubMed source PMC Full Text View journal details

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