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PMID: 6233142 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential binding of a S. cerevisiae RNA polymerase III transcription factor to two promoter segments of a tRNA gene.

The EMBO journal ·Vol. 3 ·No. 4 ·1984-04-00 ·Pages 847-53

Stillman DJ, Geiduschek EP

Abstract

A Saccharomyces cerevisiae protein fraction which binds specifically to the internal promoter regions of genes that are transcribed by RNA polymerase III is shown to function as a transcription factor. We postulate that the stable DNA binding of the factor confers stability on polymerase III transcription complexes. Analysis of the binding by DNase 'foot-printing' distinguishes three segments of the S. cerevisiae tRNALeu3 gene: a region surrounding the so-called A block of the internal promoter, a region surrounding the B block and an intermediate segment. Binding to the A and B block regions is connected, but the B block region exerts a dominant effect.

MeSH Terms
Base Sequence DNA, Fungal/metabolism DNA-Binding Proteins/metabolism DNA-Directed RNA Polymerases/metabolism Deoxyribonuclease I Endodeoxyribonucleases Operon RNA Polymerase III/metabolism RNA, Fungal/genetics RNA, Transfer, Amino Acyl/genetics Saccharomyces cerevisiae/enzymology Transcription, Genetic
Chemicals
DNA, Fungal DNA-Binding Proteins RNA, Fungal RNA, Transfer, Amino Acyl DNA-Directed RNA Polymerases RNA Polymerase III Endodeoxyribonucleases Deoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stillman D J
Geiduschek E P
References (41)
41 references, click to expand
  1. 5'-flanking sequences that inhibit in vitro transcription of a xenopus laevis tRNA gene.
    Cell. 1983 Oct;34(3):881-90 PMID: 6414719
  2. Transcription of eukaryotic tRNA genes in vitro. I. Analysis of control regions using a competition assay.
    J Biol Chem. 1983 Feb 25;258(4):2440-6 PMID: 6549757
  3. Transcription by RNA polymerase III.
    Curr Top Dev Biol. 1983;18:59-88 PMID: 6340979
  4. Nucleotide sequence of yeast LEU2 shows 5'-noncoding region has sequences cognate to leucine.
    Cell. 1982 Dec;31(2 Pt 1):319-25 PMID: 6297759
  5. Specific interactions of Saccharomyces cerevisiae proteins with a promoter region of eukaryotic tRNA genes.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6191-5 PMID: 6755466
  6. 5' flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systems.
    Cell. 1980 Nov;22(1 Pt 1):171-8 PMID: 7428038
  7. tRNA gene transcription in yeast: effects of specified base substitutions in the intragenic promoter.
    Cell. 1983 Nov;35(1):117-25 PMID: 6226365
  8. Deletion of the 3' half of the yeast tRNA-Leu3 gene does not abolish promotor function in vitro.
    Cell. 1981 Dec;27(2 Pt 1):371-9 PMID: 6277504
  9. A short 5' flanking region containing conserved sequences is required for silkworm alanine tRNA gene activity.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3416-20 PMID: 16593326
  10. Transcription of eukaryotic tRNA genes in vitro. II. Formation of stable complexes.
    J Biol Chem. 1983 Feb 25;258(4):2447-53 PMID: 6549758
  11. Mutations in PEP4 locus of Saccharomyces cerevisiae block final step in maturation of two vacuolar hydrolases.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):510-4 PMID: 6340101
  12. Promoter of a eukaryotic tRNAPro gene is composed of three noncontiguous regions.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1195-9 PMID: 6951168
  13. Sequence determinants of promoter activity.
    Cell. 1982 Oct;30(3):843-53 PMID: 6754089
  14. A detailed mutational analysis of the eucaryotic tRNAmet1 gene promoter.
    Cell. 1983 Jun;33(2):585-93 PMID: 6552939
  15. Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
    Cell. 1982 Feb;28(2):413-21 PMID: 7060135
  16. Initiation of transcription at phage T4 late promoters with purified RNA polymerase.
    Cell. 1983 Jul;33(3):887-97 PMID: 6871999
  17. The yeast tRNATyr gene intron is essential for correct modification of its tRNA product.
    Nature. 1983 Apr 21;302(5910):681-7 PMID: 6339954
  18. Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
    Cell. 1982 Apr;28(4):781-91 PMID: 7201351
  19. A tRNA gene of Xenopus laevis contains at least two sites promoting transcription.
    Nucleic Acids Res. 1979 Dec 11;7(7):1749-63 PMID: 537910
  20. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  21. The role of non-coding DNA sequences in transcription and processing of a yeast tRNA.
    Nucleic Acids Res. 1983 Sep 10;11(17):5969-88 PMID: 6351012
  22. An in vitro RNA polymerase III system from S. cerevisiae: effects of deletions and point mutations upon SUP4 gene transcription.
    Nucleic Acids Res. 1982 Dec 20;10(24):8127-43 PMID: 6298710
  23. Transcription and processing of a yeast tRNA gene containing a modified intervening sequence.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2564-8 PMID: 6994101
  24. Control region for adenovirus VA RNA transcription.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3378-82 PMID: 6943546
  25. Transcription of class III genes: formation of preinitiation complexes.
    Science. 1983 Nov 18;222(4625):740-8 PMID: 6356356
  26. Internal control regions for transcription of eukaryotic tRNA genes.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6657-61 PMID: 6947245
  27. Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.
    Nature. 1981 Dec 17;294(5842):626-31 PMID: 7312050
  28. A split promoter for a eucaryotic tRNA gene.
    Cell. 1981 May;24(2):573-85 PMID: 7237560
  29. Compilation of sequences of tRNA genes.
    Nucleic Acids Res. 1982 Jan 22;10(2):r57-81 PMID: 7063410
  30. The 5- flanking sequences of Drosophila tRNAArg genes control their in vitro transcription in a Drosophila cell extract.
    J Biol Chem. 1982 Dec 25;257(24):14738-44 PMID: 6924656
  31. Relationship between the two components of the split promoter of eukaryotic tRNA genes.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1921-5 PMID: 6952243
  32. Transcriptional control regions of the adenovirus VAI RNA gene.
    Cell. 1980 Nov;22(2 Pt 2):405-13 PMID: 7448868
  33. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
    Nucleic Acids Res. 1978 Sep;5(9):3157-70 PMID: 212715
  34. Identification of regulatory sequences contained in the 5'-flanking region of Drosophila lysine tRNA2 genes.
    J Biol Chem. 1981 Dec 10;256(23):12424-9 PMID: 6913581
  35. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
    Gene. 1979 Dec;8(1):121-33 PMID: 395029
  36. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  37. Transcription initiation of eucaryotic transfer RNA genes.
    Cell. 1982 May;29(1):3-5 PMID: 7049399
  38. The promoter sequence of a yeast tRNAtyr gene.
    Cell. 1983 Sep;34(2):655-64 PMID: 6352054
  39. Mutation in the a block of the yeast tRNAleu3 gene that allows transcription but abolishes splicing and 5'-end maturation.
    Cell. 1983 Jan;32(1):67-76 PMID: 6337723
  40. Mutations of the yeast SUP4 tRNATyr locus: transcription of the mutant genes in vitro.
    Cell. 1980 Nov;22(2 Pt 2):415-25 PMID: 7004643
  41. Specific transcription of homologous class III genes in yeast-soluble cell-free extracts.
    J Biol Chem. 1982 Jul 25;257(14):8432-41 PMID: 7045122
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-04-00
Pages
847-53
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557437
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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