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PMID: 6952243 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Relationship between the two components of the split promoter of eukaryotic tRNA genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 6 ·1982-03-00 ·Pages 1921-5

Ciliberto G, Traboni C, Cortese R

Abstract

Plasmids containing eukaryotic tRNA genes are faithfully transcribed in the nucleus of Xenopus laevis oocytes [Cortese, R., Melton, D. A., Tranquilla, T. & Smith, J. D. (1978) Nucleic Acids Res. 5, 4593-4611]. It has been established that two separated regions within the coding sequence of a tRNA gene are essential and sufficient for promotion of transcription [Hofstetter, H., Kressmann, A. & Birnstiel, M. L. (1981) Cell 24, 573-585; Ciliberto, G., Castagnoli, L., Melton, D. A. & Cortese, R. (1982) Proc. Natl. Acad. Sci. USA 79, 1195-1199]. We have constructed a hybrid tRNA gene containing one essential region from tDNALeu and the other from tDNAPro, both from Caenorhabditis elegans. This hybrid gene is efficiently transcribed, thus showing that the essential regions are independent transcriptional signals regardless of the overall regularities of the structure of tRNA genes. We have also constructed mutants of the tRNAPro gene in which the distance between the two essential regions is changed; optimal transcription occurs when this distance is about 40-50 nucleotides.

MeSH Terms
Caenorhabditis/genetics DNA, Recombinant Eukaryotic Cells/physiology Genes Leucine Mutation Operon Plasmids Proline RNA Polymerase III/metabolism RNA, Transfer/genetics Transcription, Genetic
Chemicals
DNA, Recombinant RNA, Transfer Proline RNA Polymerase III Leucine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ciliberto G
Traboni C
Cortese R
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.6.1921
Published
1982-03-00
Pages
1921-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346093
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.6.1921 PMC Full Text View journal details
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