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PMID: 6396084 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The myc proteins are not associated with chromatin in mitotic cells.

The EMBO journal ·Vol. 3 ·No. 12 ·1984-12-01 ·Pages 2947-50

Winqvist R, Saksela K, Alitalo K

Abstract

The protein products of cellular and viral myc oncogenes are detected in nuclei by immunofluorescence. No myc fluorescence is found in nucleoli. In mitotic cells the myc antigens are not found associated with metaphase chromosomes, but are diffusely distributed throughout the cytoplasm. Cytoplasmic myc fluorescence is first observed when chromatin begins to condense in early prophase. Granular nuclear myc fluorescence is again discerned in telophase cells, when the nuclear envelope is formed and becomes more prominent upon cytokinesis; concomitantly the diffuse cytoplasmic myc staining is lost. These results suggest that myc proteins not only bind to DNA or chromatin, but are also associated with other structural systems in the nuclei.

MeSH Terms
Animals Cell Line Cell Nucleus/ultrastructure Chick Embryo Chromatin/ultrastructure DNA, Neoplasm/analysis Embryo, Nonmammalian Fluorescent Antibody Technique Genes, Viral Mitosis Neoplasm Proteins/analysis Oncogenes Quail Retroviridae/genetics
Chemicals
Chromatin DNA, Neoplasm Neoplasm Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Winqvist R
Saksela K
Alitalo K
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39 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-12-01
Pages
2947-50
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557794
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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