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PMID: 6401705 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Adaptive response of Bacillus subtilis to N-methyl-N'-nitro-N-nitrosoguanidine.

Journal of bacteriology ·Vol. 153 ·No. 2 ·1983-02-00 ·Pages 756-62

Hadden CT, Foote RS, Mitra S

Abstract

Cell extracts of Bacillus subtilis contain a methyltransferase that appears to remove the O6-methyl group from O6-methylguanine in DNA in situ. This reaction proceeds in a stoichiometric fashion, as in Escherichia coli. However, the basal level of the enzyme (approximately 240 molecules per cell) is significantly higher in B. subtilis than in E. coli. In addition, the methyltransferase level increases by an order of magnitude as a result of de novo protein synthesis after adaptive treatment with a low concentration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), as in E. coli. Concomitant with adaptation, B. subtilis cells become more resistant to both killing and mutagenesis by a challenge dose of N-methyl-N'-nitro-N-nitrosoguanidine. We present evidence to support the hypothesis that the majority of N-methyl-N'-nitro-N-nitrosoguanidine-induced mutations in B. subtilis are of the guanine-to-adenine transition type.

MeSH Terms
Adaptation, Physiological Bacillus subtilis/drug effects,enzymology,genetics Drug Resistance, Microbial Enzyme Induction Guanine/analogs & derivatives,metabolism Methylnitronitrosoguanidine/pharmacology Methyltransferases/biosynthesis Mutation/drug effects O(6)-Methylguanine-DNA Methyltransferase
Chemicals
Methylnitronitrosoguanidine Guanine O-(6)-methylguanine Methyltransferases O(6)-Methylguanine-DNA Methyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hadden C T
Foote R S
Mitra S
References (33)
33 references, click to expand
  1. Adaptive response to alkylating agents involves alteration in situ of O6-methylguanine residues in DNA.
    Nature. 1979 Jul 5;280(5717):76-7 PMID: 15305585
  2. Induction of a DNA glycosylase for N-methylated purines is part of the adaptive response to alkylating agents.
    Nature. 1982 Apr 22;296(5859):770-3 PMID: 7040983
  3. Mutagenesis of the replication point by nitrosoguanidine: map and pattern of replication of the Escherichia coli chromosome.
    J Mol Biol. 1968 May 14;33(3):705-19 PMID: 4882616
  4. Suppressor system in Bacillus subtilis 168.
    J Bacteriol. 1969 Mar;97(3):1397-402 PMID: 4975748
  5. Possible relevance of O-6 alkylation of deoxyguanosine to the mutagenicity and carcinogenicity of nitrosamines and nitrosamides.
    Nature. 1969 Jul 12;223(5202):206-7 PMID: 5791738
  6. Studies on the mutagenic action of N-methyl-N'-nitro-N-nitrosoguanidine in Paramecium aurella with emphasis on repair processes.
    Mutat Res. 1970 Mar;9(3):261-71 PMID: 5480471
  7. Induction of closely linked multiple mutations by nitrosoguanidine.
    Nat New Biol. 1971 Mar 24;230(12):122-5 PMID: 4927375
  8. On the process of cellular division in Escherichia coli. I. Asymmetrical cell division and production of deoxyribonucleic acid-less bacteria.
    J Mol Biol. 1968 Jul 14;35(1):175-92 PMID: 4939776
  9. Evidence suggestive of compartmentalization of deoxyribonucleic acid-synthesizing systems in freeze-treated Bacillus subtilis.
    J Bacteriol. 1971 Dec;108(3):1250-6 PMID: 5003175
  10. Gene frequency analysis of chromosomal initiation sites in Bacillus subtilis after ultraviolet light or x-ray exposure.
    J Bacteriol. 1972 Jan;109(1):379-84 PMID: 4621630
  11. Quantitation of the adaptive response to alkylating agents.
    Nature. 1979 Jul 5;280(5717):74-6 PMID: 15305584
  12. Mutations induced in Haemophilus influenzae by transformation with nitrosoguanidine-treated DNA.
    Mutat Res. 1972 Feb;14(2):137-46 PMID: 4335556
  13. Mutagen stability of alkylation-sensitive mutants of Bacillus subtilis.
    J Bacteriol. 1972 Apr;110(1):47-55 PMID: 4622905
  14. Gene-enzyme relationships of aromatic acid biosynthesis in Bacillus subtilis.
    J Bacteriol. 1973 Oct;116(1):59-66 PMID: 4200844
  15. Mutation fixation in MNNG-treated Haemophilus influenzae as determined by transformation.
    Mutat Res. 1974 Jan;22(1):1-14 PMID: 4546326
  16. Involvement of DNA replication and repair in mutagenesis of Haemophilus influenzae induced by N-nitrosocarbaryl.
    Mutat Res. 1974 Aug;24(2):105-15 PMID: 4546840
  17. Competent Bacillus subtilis cultures synthesize a denatured DNA binding activity.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):323-7 PMID: 164019
  18. A simple procedure for localized mutagenesis using nitrosoguanidine.
    Mol Gen Genet. 1974;134(1):77-83 PMID: 4617157
  19. Identification of coreplicating chromosomal sectors in Bacillus subtilis by nitrosoguanidine-induced comutation.
    J Bacteriol. 1976 Mar;125(3):755-61 PMID: 815245
  20. Identifying sites of simultaneous DNA replication in eukaryotes by N-methyl-N'-nitro-N-nitrosoguanidine multiple mutagenesis.
    Mol Gen Genet. 1977 Mar 28;152(1):53-7 PMID: 325387
  21. A new pathway for DNA repair in Escherichia coli.
    Nature. 1977 May 19;267(5608):281-3 PMID: 325420
  22. An adaptive response of E. coli to low levels of alkylating agent: comparison with previously characterised DNA repair pathways.
    Mol Gen Genet. 1977 Nov 29;157(1):1-9 PMID: 414071
  23. Genetic studies of the lac repressor. IV. Mutagenic specificity in the lacI gene of Escherichia coli.
    J Mol Biol. 1977 Dec 15;117(3):577-606 PMID: 416218
  24. Enchancement of nitrosoguanidine mutagenesis by chloramphenicol in Escherichia coli K-12.
    J Bacteriol. 1978 Oct;136(1):460-2 PMID: 361705
  25. Repair of O6-methylguanine in adapted Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6017-20 PMID: 282622
  26. Enhancement of deoxyribonucleic acid polymerase I-directed repair synthesis in toluene-treated Escherichia coli after growth in the presence of low levels of N-methyl-N'-nitro-N-nitrosoguanidine.
    J Bacteriol. 1979 Mar;137(3):1439-42 PMID: 374353
  27. Adaptation to alkylation resistance involves the induction of a DNA glycosylase.
    Nature. 1982 Apr 22;296(5859):773-5 PMID: 7040984
  28. Two DNA glycosylases in Escherichia coli which release primarily 3-methyladenine.
    Biochemistry. 1982 Mar 16;21(6):1162-9 PMID: 7041972
  29. O6-methylguanine-DNA methyltransferase in wild-type and ada mutants of Escherichia coli.
    J Bacteriol. 1982 Oct;152(1):534-7 PMID: 6749819
  30. Repair of alkylated DNA in Escherichia coli. Methyl group transfer from O6-methylguanine to a protein cysteine residue.
    J Biol Chem. 1980 Nov 25;255(22):10569-71 PMID: 7000780
  31. Transformation and transduction in recombination-defective mutants of Bacillus subtilis.
    J Bacteriol. 1967 Jun;93(6):1925-37 PMID: 4960898
  32. Demethylation of O6-methylguanine in a synthetic DNA polymer by an inducible activity in Escherichia coli.
    Biochem Biophys Res Commun. 1980 Nov 28;97(2):654-9 PMID: 7008792
  33. Further studies on the induction of mutation in Haemophilus influenzae by N-methyl-N'-nitro-N-nitrosoguanidine: lack of an inducible error-free repair system and the effect of exposure medium.
    Mutat Res. 1980 Aug;72(3):361-72 PMID: 6969848
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.153.2.756-762.1983
Published
1983-02-00
Pages
756-62
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221694
Subset
IM
Grants
NCI NIH HHS · Y01-CP-00200 · United States
External Links
PubMed source DOI: 10.1128/jb.153.2.756-762.1983 PMC Full Text View journal details
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