Home LiteratureArticle Details
PMID: 6407007 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Diversity and joining segments of mouse immunoglobulin heavy chain genes are closely linked and in the same orientation: implications for the joining mechanism.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 10 ·1983-05-00 ·Pages 3030-4

Wood C, Tonegawa S

Abstract

We have mapped 12 diversity (D) gene segments of mouse Ig heavy chains between 80 and 1 kilobases 5' to the four joining (JH) gene segments by molecular analysis of DNA clones isolated from recombinant phage and cosmid libraries. All identified D and JH segments are in the same 5'-3' orientation. The significance of these findings with respect to the joining mechanism for Ig gene segments is discussed.

MeSH Terms
Animals Antibody Diversity Base Sequence Chromosome Mapping Genetic Linkage Immunoglobulin Constant Regions/genetics Immunoglobulin Heavy Chains/genetics Mice Recombination, Genetic
Chemicals
Immunoglobulin Constant Regions Immunoglobulin Heavy Chains
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wood C
Tonegawa S
References (24)
24 references, click to expand
  1. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  2. Cloning of an immunoglobulin variable region gene from mouse embryo.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3518-22 PMID: 410021
  3. DNA clones containing mouse immunoglobulin kappa chain genes isolated by in vitro packaging into phage lambda coats.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4709-13 PMID: 105355
  4. Sequences at the somatic recombination sites of immunoglobulin light-chain genes.
    Nature. 1979 Jul 26;280(5720):288-94 PMID: 111144
  5. Amino acid sequence of homogeneous antibodies to dextran and DNA rearrangements in heavy chain V-region gene segments.
    Nature. 1980 Jan 3;283(5742):35-40 PMID: 6765983
  6. Exon shuffling generates an immunoglobulin heavy chain gene.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):2138-42 PMID: 6769120
  7. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
    Cell. 1980 Apr;19(4):981-92 PMID: 6769593
  8. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
  9. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  10. A rearranged DNA sequence possibly related to the translocation of immunoglobulin gene segments.
    Nucleic Acids Res. 1980 Apr 25;8(8):1709-20 PMID: 6253944
  11. Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes.
    Nature. 1981 Apr 16;290(5807):562-5 PMID: 6783961
  12. Identification of D segments of immunoglobulin heavy-chain genes and their rearrangement in T lymphocytes.
    Nature. 1981 Apr 16;290(5807):565-70 PMID: 6783962
  13. Linkage of the four gamma subclass heavy chain genes.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):474-8 PMID: 6264445
  14. Isolation of beta-globin-related genes from a human cosmid library.
    Gene. 1981 Apr;13(3):227-37 PMID: 6266915
  15. Rapid and efficient cosmid cloning.
    Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 PMID: 6269067
  16. Mapping of immunoglobulin variable region genes: relationship to the 'deletion' model of immunoglobulin gene rearrangement.
    Nucleic Acids Res. 1981 Nov 11;9(21):5725-35 PMID: 6796941
  17. Organization, structure, and assembly of immunoglobulin heavy chain diversity DNA segments.
    J Exp Med. 1982 Jan 1;155(1):201-18 PMID: 6798155
  18. The mechanism of cytoplasmic orthopoxvirus DNA replication.
    Cell. 1981 Dec;27(2 Pt 1):391-401 PMID: 6277506
  19. Recombined flanks of the variable and joining segments of immunoglobulin genes.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1383-7 PMID: 6803241
  20. Organization of the constant-region gene family of the mouse immunoglobulin heavy chain.
    Cell. 1982 Mar;28(3):499-506 PMID: 6804095
  21. DNA between variable and joining gene segments of immunoglobulin kappa light chain is frequently retained in cells that rearrange the kappa locus.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):262-6 PMID: 6804938
  22. Sets of immunoglobulin V kappa genes homologous to ten cloned V kappa sequences: implications for the number of germline V kappa genes.
    J Mol Appl Genet. 1981;1(2):103-16 PMID: 6286818
  23. Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4118-22 PMID: 6287467
  24. Continuing kappa-gene rearrangement in a cell line transformed by Abelson murine leukemia virus.
    Cell. 1982 Oct;30(3):807-16 PMID: 6291785
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.10.3030
Published
1983-05-00
Pages
3030-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393967
Subset
IM
Grants
NIAID NIH HHS · AI-17879-02 · United States
NCI NIH HHS · CA-14051 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.10.3030 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2