Home LiteratureArticle Details
PMID: 6415036 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transcriptional regulation of several genes for bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata in response to oxygen.

Journal of bacteriology ·Vol. 156 ·No. 2 ·1983-11-00 ·Pages 686-94

Biel AJ, Marrs BL

Abstract

Although it has been shown that bacteriochlorophyll synthesis in Rhodopseudomonas capsulata is repressed by oxygen and high light intensity, few details of regulation by these environmental factors are known, primarily owing to a lack of assays for the biosynthetic enzymes. We have examined regulation at the transcriptional level by isolating and studying fusions between the Mu d1(Apr lac) phage and various bch genes. In these strains, the lacZ gene of the phage is under the control of bch gene promoters. We have found that atmospheric oxygen tension (20% O2) reduces the expression of these fusions at least twofold compared with low oxygen tension (2% O2). Therefore, transcription of the bchA, bchB, bchC, bchG, and bchH genes is regulated in response to oxygen.

MeSH Terms
Bacteriochlorophylls/genetics,isolation & purification Chlorophyll/analogs & derivatives Escherichia coli/genetics Genes/drug effects Genes, Bacterial/drug effects Genotype Oxygen/pharmacology Plasmids Porphyrins/isolation & purification Rhodopseudomonas/drug effects,genetics Species Specificity Transcription, Genetic/drug effects beta-Galactosidase/genetics
Chemicals
Bacteriochlorophylls Porphyrins Chlorophyll beta-Galactosidase Oxygen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Biel A J
Marrs B L
References (20)
20 references, click to expand
  1. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530-3 PMID: 159458
  2. Mobilization of the genes for photosynthesis from Rhodopseudomonas capsulata by a promiscuous plasmid.
    J Bacteriol. 1981 Jun;146(3):1003-12 PMID: 6787008
  3. Expression of the transposable lac operon Tn951 in Rhodopseudomonas sphaeroides.
    J Bacteriol. 1982 Nov;152(2):924-7 PMID: 6290458
  4. R-prime site-directed transposon Tn7 mutagenesis of the photosynthetic apparatus in Rhodopseudomonas capsulata.
    J Mol Biol. 1982 Nov 25;162(1):17-41 PMID: 6296403
  5. Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique.
    J Bacteriol. 1983 May;154(2):580-90 PMID: 6302077
  6. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.
    J Bacteriol. 1951 Sep;62(3):293-300 PMID: 14888646
  7. Kinetic studies of pigment synthesis by non-sulfur purple bacteria.
    J Cell Physiol. 1957 Feb;49(1):25-68 PMID: 13416343
  8. The accumulation of bacteriochlorophyll precursors by mutant and wild-type strains of Rhodopseudomonas spheroides.
    Biochem J. 1966 Jul;100(1):175-83 PMID: 5965250
  9. The biosynthesis of bacteriochlorophyll. The characterization of latter stage intermediates from mutants of Rhodopseudomonas spheroides.
    Biochemistry. 1969 Aug;8(8):3473-82 PMID: 4309211
  10. Magnesium protoporphyrin chelatase activity in Rhodopseudomonas spheroides. Studies with whole cells.
    Biochem J. 1972 Mar;127(1):97-106 PMID: 4627449
  11. Control of magnesium-protoporphyrin chelatase activity in Rhodopseudomonas spheroides. Role of light, oxygen, and electron and energy transfer.
    Biochem J. 1973 Aug;134(4):833-45 PMID: 4202754
  12. The regulation of heme and chlorophyll synthesis in bacteria.
    Ann N Y Acad Sci. 1975 Apr 15;244:334-47 PMID: 166593
  13. A possible alternate pathway of bacteriochlorophyll biosynthesis in a mutant of Rhodopseudomonas sphaeroides.
    Biochemistry. 1975 Jul 15;14(14):3132-7 PMID: 1080053
  14. Characterization of Rhodopseudomonas capsulata.
    Arch Microbiol. 1975 Nov 7;105(3):207-16 PMID: 1103769
  15. Isolation of nonsense suppressor mutants in Pseudomonas.
    J Bacteriol. 1976 Apr;126(1):177-82 PMID: 816771
  16. Map of genes for carotenoid and bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata.
    J Bacteriol. 1976 May;126(2):619-29 PMID: 1262313
  17. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  18. Plasmid RP4, with Escherichia coli DNA inserted in vitro, mediates chromosomal transfer.
    Plasmid. 1979 Jan;2(1):130-6 PMID: 382198
  19. Positive control of ilvC expression in Escherichia coli K-12; identification and mapping of regulatory gene ilvY.
    J Bacteriol. 1979 Sep;139(3):1014-20 PMID: 113381
  20. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.156.2.686-694.1983
Published
1983-11-00
Pages
686-94
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217884
Subset
IM
Grants
NIGMS NIH HHS · GM 20173 · United States
NHLBI NIH HHS · HL 07050 · United States
External Links
PubMed source DOI: 10.1128/jb.156.2.686-694.1983 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2