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PMID: 6416834 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Analysis of antibody diversity: V-D-J mRNA nucleotide sequence of four anti-GAT monoclonal antibodies. A paucigene system using alternate D-J recombinations to generate functionally similar hypervariable regions.

The EMBO journal ·Vol. 2 ·No. 6 ·1983-00-00 ·Pages 867-72

Rocca-Serra J, Matthes HW, Kaartinen M, Milstein C, Thèze J, Fougereau M

Abstract

The nucleotide sequence of four anti-(Glu60-Ala30-Tyr10)n (GAT) monoclonal gamma 1 heavy chain mRNAs was determined from codon 10 to 120. This sequence overlaps with the NH2-terminal amino acid sequence, allowing elucidation of the complete protein sequence encompassing regions VH, D and JH. These sequences, which are highly conserved, indicate that anti-GAT antibodies expressing the same public idiotypic specificities represent a paucigene system, which uses at least two D-J combinations leading to functionally similar hypervariable regions involved in the recognition of the dominant Glu-Tyr determinant. D regions are encoded by D genes which are closely related either to the D-SP2 or the D.FL16 germ line gene cores.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal/genetics Antibody Diversity Base Sequence Genes Immunoglobulin Heavy Chains/genetics Immunoglobulin Idiotypes/genetics Immunoglobulin Variable Region/genetics Mice Mice, Inbred BALB C RNA, Messenger/genetics Recombination, Genetic
Chemicals
Antibodies, Monoclonal Immunoglobulin Heavy Chains Immunoglobulin Idiotypes Immunoglobulin Variable Region RNA, Messenger anti-GAT antibodies, idiotypic
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rocca-Serra J
Matthes H W
Kaartinen M
Milstein C
Thèze J
Fougereau M
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43 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
867-72
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555202
Subset
IM
Databases
GENBANK
K00687, K02105, K02106, K02107
External Links
PubMed source PMC Full Text View journal details
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